Histochemistry of lactic dehydrogenase in heart and pectoralis muscles of rat - PubMed (original) (raw)

Histochemistry of lactic dehydrogenase in heart and pectoralis muscles of rat

N Baba et al. J Cell Biol. 1971 Dec.

Abstract

Left-ventricular heart muscle and pectoralis major muscle of the rat were studied to determine the intracellular localization of lactic dehydrogenase (LDH) isoenzymes. Fixation of tissue for 2 hr in 2% buffered formaldehyde provided the best preservation of the ultrastructure and enzyme activity. Total LDH activity was found diffusely in the ground substance of the sarcoplasm and in the mitochondria of the heart muscle. In skeletal muscle a strong reaction was noted in the sarcoplasmic reticulum, and moderate activity was seen in the ground substance of the sarcoplasm and in the mitochondria. Differentiation of the isoenzymes of LDH was accomplished by addition of 4 M urea or application of heat. Heart-type isoenzymes were mainly localized in the mitochondria and sarcoplasm, whereas muscle-type isoenzymes were localized mainly in the sarcoplasmic reticulum of the skeletal muscle. It is speculated that the sarcoplasmic reticulum of the skeletal muscle is the site of anaerobic glycolysis and that the sarcoplasm and mitochondria are involved primarily in aerobic metabolism of pyruvate.

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References

1. 1. J Histochem Cytochem. 1964 Nov;12:797-804 - PubMed
2. 1. Science. 1965 Oct 15;150(3694):364-6 - PubMed
3. 1. J Biophys Biochem Cytol. 1958 Nov 25;4(6):753-60 - PubMed
4. 1. Science. 1963 Nov 15;142(3594):965-7 - PubMed
5. 1. Clin Chim Acta. 1970 Feb;27(2):225-32 - PubMed

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