Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease - PubMed (original) (raw)
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease
J M Chirgwin et al. Biochemistry. 1979.
Abstract
Intact ribonucleic acid (RNA) has been prepared from tissues rich in ribonuclease such as the rat pancreas by efficient homogenization in a 4 M solution of the potent protein denaturant guanidinium thiocyanate plus 0.1 M 2-mercaptoethanol to break protein disulfide bonds. The RNA was isolated free of protein by ethanol precipitation or by sedimentation through cesium chloride. Rat pancreas RNA obtained by these means has been used as a source for the purification of alpha-amylase messenger ribonucleic acid.
Similar articles
- Ribonucleic acid isolated by cesium chloride centrifugation.
Glisin V, Crkvenjakov R, Byus C. Glisin V, et al. Biochemistry. 1974 Jun 4;13(12):2633-7. doi: 10.1021/bi00709a025. Biochemistry. 1974. PMID: 4831907 No abstract available. - The isolation of eukaryotic messenger RNA.
Taylor JM. Taylor JM. Annu Rev Biochem. 1979;48:681-717. doi: 10.1146/annurev.bi.48.070179.003341. Annu Rev Biochem. 1979. PMID: 382995 Review. No abstract available. - Purification of myosin translational control RNA and its interaction with myosin messenger RNA.
Heywood SM, Kennedy DS. Heywood SM, et al. Biochemistry. 1976 Jul 27;15(15):3314-9. doi: 10.1021/bi00660a023. Biochemistry. 1976. PMID: 986157 - Methods of RNA purification. All ways (should) lead to Rome.
Vomelová I, Vanícková Z, Sedo A. Vomelová I, et al. Folia Biol (Praha). 2009;55(6):243-51. Folia Biol (Praha). 2009. PMID: 20163774 Review.
Cited by
- Total RNA Extraction from Rice Vegetative Tissues Using Magnetic Beads.
Yoshino K, Kawakatsu T. Yoshino K, et al. Methods Mol Biol. 2025;2869:7-13. doi: 10.1007/978-1-0716-4204-7_2. Methods Mol Biol. 2025. PMID: 39499462 - Agave-Laurate-Bioconjugated Fructans Decrease Hyperinsulinemia and Insulin Resistance, Whilst Increasing IL-10 in Rats with Metabolic Syndrome Induced by a High-Fat Diet.
González-Garibay AS, Sandoval G, Torres-González OR, Bastidas-Ramírez BE, Sánchez-Hernández IM, Padilla-Camberos E. González-Garibay AS, et al. Pharmaceuticals (Basel). 2024 Aug 6;17(8):1036. doi: 10.3390/ph17081036. Pharmaceuticals (Basel). 2024. PMID: 39204141 Free PMC article. - The endogenous Mtv8 locus and the immunoglobulin repertoire.
Beilinson HA, Erickson SA, Golovkina T. Beilinson HA, et al. Front Immunol. 2024 Mar 5;15:1345467. doi: 10.3389/fimmu.2024.1345467. eCollection 2024. Front Immunol. 2024. PMID: 38504980 Free PMC article. - Assessment of Strategies for Preserving Swine Viral RNA Targets in Diagnostic Specimens.
Munguía-Ramírez B, Giménez-Lirola L, Zimmerman J. Munguía-Ramírez B, et al. Microorganisms. 2024 Feb 18;12(2):410. doi: 10.3390/microorganisms12020410. Microorganisms. 2024. PMID: 38399814 Free PMC article. Review. - Changes in the Composition of Unstimulated and Stimulated Saliva Due to Chewing Sour Cherry Gum and a Toothbrush Change.
Skopkó BE, Homoki JR, Fazekas MÉ, Paholcsek M, Fauszt P, Dávid P, Stündl L, Molnár PB, Forgács IN, Váradi J, Bágyi KÁ, Remenyik J. Skopkó BE, et al. Cells. 2024 Jan 29;13(3):251. doi: 10.3390/cells13030251. Cells. 2024. PMID: 38334643 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources