Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands - PubMed (original) (raw)

Evidence for a single-stranded adenovirus-associated virus genome: isolation and separation of complementary single strands

K I Berns et al. J Virol. 1970 Jun.

Abstract

Single-stranded adenovirus-associated virus type 2 deoxyribonucleic acid (AAV-2 DNA) has been isolated from the virion after enzymatic pretreatment of the particles by heating at 53 C for 1 hr in 0.015 m NaCl plus 0.0015 m sodium citrate in the presence of 1% sodium dodecyl sulfate. Double-stranded AAV-2 DNA present as a marker is not denatured by this treatment. AAV-2 single-stranded DNA is composed of two complementary species which can be separated in neutral CsCl when 5-bromodeoxyuridine has been substituted for thymidine in the DNA. The present report is the first documented instance of the separation of complementary strands of an animal virus DNA.

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