Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis - PubMed (original) (raw)

Separation of ten reovirus genome segments by polyacrylamide gel electrophoresis

A J Shatkin et al. J Virol. 1968 Oct.

Abstract

Double-stranded ribonucleic acid (RNA) extracted from purified reoviruses of all three serotypes and from type 3 virus-infected cells was analyzed by polyacrylamide gel electrophoresis. It was calculated that each RNA includes 10 segments: 3 large, 3 intermediate, and 4 small fragments corresponding to molecular weights of about 2.5, 1.4, and 0.8 x 10(6) daltons, respectively, or a total of 15 x 10(6) daltons.

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