Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters - PubMed (original) (raw)
Cell interactions in the primary immune response in vitro: a requirement for specific cell clusters
D E Mosier. J Exp Med. 1969.
Abstract
Mouse spleen cells were found to associate in cell clusters during the primary immune response to sheep erythrocytes in vitro. About 10% of the cell clusters had the following unique properties; (a) they contained most, if not all, antibody-forming cells, (b) they contained only cells forming antibody to one antigen when cell cultures were immunized with two antigens, (c) the cells in clusters reaggregated specifically after dispersion, and (d) the specific reaggregation of clusters appeared to be blocked by antibody to the antigen. The integrity of cell clusters was required for the proliferation of antibody-forming cells, and prevention of clustering by mechanical means or by excess antibody blocked the immune response. Antibody and antigenic determinants on the surfaces of cells probably provide the basis for interaction. The unique microenvironment of cell clusters was essential for the primary immune response in vitro.
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References
- Nature. 1968 Jun 15;218(5146):1078-9 - PubMed
- J Exp Med. 1965 Aug 1;122:423-40 - PubMed
- Proc Natl Acad Sci U S A. 1968 Oct;61(2):542-7 - PubMed
- J Exp Med. 1967 Aug 1;126(2):223-34 - PubMed
- Nature. 1968 Jun 8;218(5145):972-3 - PubMed