Electrical properties of Neurospora crassa. Effects of external cations on the intracellular potential - PubMed (original) (raw)
Electrical properties of Neurospora crassa. Effects of external cations on the intracellular potential
C L Slayman. J Gen Physiol. 1965 Sep.
Abstract
Glass micropipette electrodes have been employed to study the transsurface potential difference of Neurospora crassa. For mature hyphae grown in agar cultures, the internal potential is large and negative, often exceeding -200 mv. The potential is sensitive to the concentrations of extracellular potassium, sodium, hydrogen, and calcium ions, but does not vary in a manner which is readily explained by ionic diffusion potentials. With extracellular solutions containing only potassium chloride (or sulfate) and sucrose, the internal potential shifts toward zero (becomes less negative) at 45 mv per tenfold increase of potassium, over the range 0.1 to 10 mM. A similar result has been found with sodium, though the slope is only 33 mv/log unit. Calcium (1 mM) diminishes the influence of potassium and sodium by 60 to 70 per cent. As potassium or sodium is raised above 20 mM, the slope of the internal potential increases sharply to 85 to 90 mv/log unit, both in the presence and absence of calcium. With increasing hydrogen ion concentration, too, the internal potential shifts toward zero; in this case the slope is about 12 mv/pH unit at pH 9 and rises smoothly to 33 mv/pH unit at pH 3. All these phenomena are probably properties of the plasma membrane. The polysaccharide cell wall contains few fixed negative charges, has a low transverse resistance, and supports very little potential difference when separated from the plasma membrane.
Similar articles
- Electrical properties of Neurospora crassa. Respiration and the intracellular potential.
Slayman CL. Slayman CL. J Gen Physiol. 1965 Sep;49(1):93-116. doi: 10.1085/jgp.49.1.93. J Gen Physiol. 1965. PMID: 5862508 Free PMC article. - Transcellular ion currents and extension of Neurospora crassa hyphae.
Takeuchi Y, Schmid J, Caldwell JH, Harold FM. Takeuchi Y, et al. J Membr Biol. 1988;101(1):33-41. doi: 10.1007/BF01872817. J Membr Biol. 1988. PMID: 2966862 - Charges and potentials at the nerve surface. Divalent ions and pH.
Hille B. Hille B. J Gen Physiol. 1968 Feb;51(2):221-36. doi: 10.1085/jgp.51.2.221. J Gen Physiol. 1968. PMID: 5641636 Free PMC article. - Ionic regulation of the plasma membrane potential of rainbow trout (Salmo gairdneri) spermatozoa: role in the initiation of sperm motility.
Gatti JL, Billard R, Christen R. Gatti JL, et al. J Cell Physiol. 1990 Jun;143(3):546-54. doi: 10.1002/jcp.1041430320. J Cell Physiol. 1990. PMID: 2358473 - Static material properties of the tectorial membrane: a summary.
Freeman DM, Masaki K, McAllister AR, Wei JL, Weiss TF. Freeman DM, et al. Hear Res. 2003 Jun;180(1-2):11-27. doi: 10.1016/s0378-5955(03)00072-8. Hear Res. 2003. PMID: 12782349 Review.
Cited by
- Cation transport and electrogenesis by Streptococcus faecalis. I. The membrane potential.
Harold FM, Papineau D. Harold FM, et al. J Membr Biol. 1972;8(1):27-44. doi: 10.1007/BF01868093. J Membr Biol. 1972. PMID: 4628384 No abstract available. - The proton pumps of the plasmalemma and the tonoplast of higher plants.
Marrè E, Ballarin-Denti A. Marrè E, et al. J Bioenerg Biomembr. 1985 Feb;17(1):1-21. doi: 10.1007/BF00744985. J Bioenerg Biomembr. 1985. PMID: 2859282 Review. - Depolarization of the plasma membrane of Neurospora during active transport of glucose: evidence for a proton-dependent cotransport system.
Slayman CL, Slayman CW. Slayman CL, et al. Proc Natl Acad Sci U S A. 1974 May;71(5):1935-9. doi: 10.1073/pnas.71.5.1935. Proc Natl Acad Sci U S A. 1974. PMID: 4525303 Free PMC article. - The effects of bicarbonate ions and external pH on the membrane potential and resistance ofNitella translucens.
Spanswick RM. Spanswick RM. J Membr Biol. 1970 Dec;2(1):59-70. doi: 10.1007/BF01869850. J Membr Biol. 1970. PMID: 24174137 - Circulation of potassium across the plasma membrane of Blastocladiella emersonii: K+ channel.
Van Brunt J, Caldwell JH, Harold FM. Van Brunt J, et al. J Bacteriol. 1982 Jun;150(3):1449-61. doi: 10.1128/jb.150.3.1449-1461.1982. J Bacteriol. 1982. PMID: 6281245 Free PMC article.
References
- J Bacteriol. 1959 Oct;78:550-6 - PubMed
- Fed Proc. 1951 Sep;10(3):611-21 - PubMed
- J Physiol. 1956 Nov 28;134(2):278-310 - PubMed
- Science. 1959 Feb 27;129(3348):572 - PubMed
- J Biol Chem. 1951 Oct;192(2):755-67 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials