The nitrendipine-sensitive Ca2+ channel in chick muscle cells and its appearance during myogenesis in vitro and in vivo - PubMed (original) (raw)
. 1984 Sep 25;259(18):11366-72.
- PMID: 6088541
Free article
The nitrendipine-sensitive Ca2+ channel in chick muscle cells and its appearance during myogenesis in vitro and in vivo
A Schmid et al. J Biol Chem. 1984.
Free article
Abstract
The nitrendipine-sensitive Ca2+ channel of chick skeletal myotubes in culture has been studied using both the 45Ca2+ flux technique and [3H]nitrendipine binding experiments. Ca2+ uptake is insensitive to nitrendipine when chick myotubes in culture are polarized. Whereas depolarization reveals a new component of 45Ca2+ influx which is inhibited by nitrendipine. Half-maximal inhibition occurs at a nitrendipine concentration of 0.7 nM. This value is similar to the dissociation constant Kd = 0.4 nM found in [3H]nitrendipine binding experiments. During myogenesis in vitro the nitrendipine receptor is absent in myoblasts and appears in parallel with the fusion process. Two stages of increased binding have been observed in vivo. The first one, which occurs during embryonic life, has the same properties as in the in vitro development. The second stage occurs near hatching and corresponds to a large increase in the number of nitrendipine receptors. This increase is accompanied by a decrease of affinity of nitrendipine for its receptor by a factor of 4 to 10. Chronic denervation produces a further increase in the number of nitrendipine receptors which reaches a factor of about 2 at 15 days of denervation. Results are discussed in relation to the particular localization of these channels in transverse tubules and with the innervation.
Similar articles
- Differentiation of receptor sites for [3H]nitrendipine in chick hearts and physiological relation to the slow Ca2+ channel and to excitation-contraction coupling.
Renaud JF, Kazazoglou T, Schmid A, Romey G, Lazdunski M. Renaud JF, et al. Eur J Biochem. 1984 Mar 15;139(3):673-81. doi: 10.1111/j.1432-1033.1984.tb08056.x. Eur J Biochem. 1984. PMID: 6321185 - Dihydropyridine-sensitive Ca2+ channels: molecular properties of interaction with Ca2+ channel blockers, purification, subunit structure, and differentiation.
Lazdunski M, Barhanin J, Borsotto M, Fosset M, Galizzi JP, Renaud JF, Romey G, Schmid A. Lazdunski M, et al. J Cardiovasc Pharmacol. 1986;8 Suppl 8:S13-9. J Cardiovasc Pharmacol. 1986. PMID: 2433514
Cited by
- Extracellular Ca(2+)-dependent and independent calcium transient in fetal myotubes.
Strube C, Beurg M, Georgescauld D, Bournaud R, Shimahara T. Strube C, et al. Pflugers Arch. 1994 Jul;427(5-6):517-23. doi: 10.1007/BF00374269. Pflugers Arch. 1994. PMID: 7971150 - Developmental induction of DHPR alpha 1s and RYR1 gene expression does not require neural or mechanical signals.
Radzyukevich TL, Cougnon MH, Moseley AE, Heiny JA. Radzyukevich TL, et al. J Muscle Res Cell Motil. 2004;25(1):87-94. doi: 10.1023/b:jure.0000021351.41103.ad. J Muscle Res Cell Motil. 2004. PMID: 15160492 - Coexistence of two classes of glibenclamide-inhibitable ATP-regulated K+ channels in avian skeletal muscle.
Fosset M, Allard B, Lazdunski M. Fosset M, et al. Pflugers Arch. 1995 Nov;431(1):117-24. doi: 10.1007/BF00374384. Pflugers Arch. 1995. PMID: 8584408 - Calcium channels: molecular pharmacology, structure and regulation.
Hosey MM, Lazdunski M. Hosey MM, et al. J Membr Biol. 1988 Sep;104(2):81-105. doi: 10.1007/BF01870922. J Membr Biol. 1988. PMID: 2903935 Review. No abstract available. - Plasticity of the transverse tubules following denervation and subsequent reinnervation in rat slow and fast muscle fibres.
Takekura H, Tamaki H, Nishizawa T, Kasuga N. Takekura H, et al. J Muscle Res Cell Motil. 2003;24(7):439-51. doi: 10.1023/a:1027356912404. J Muscle Res Cell Motil. 2003. PMID: 14677647
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous