Sequence requirements for nuclear location of simian virus 40 large-T antigen - PubMed (original) (raw)

. 1984 Sep;311(5981):33-8.

doi: 10.1038/311033a0.

• PMID: 6088992
• DOI: 10.1038/311033a0

Sequence requirements for nuclear location of simian virus 40 large-T antigen

D Kalderon et al. Nature. 1984 Sep.

Abstract

A point mutation in the simian virus 40 large-T gene, which was generated by mixed oligonucleotide mutagenesis and resulted in the conversion of Lys 128 to Thr, produced a large-T antigen that was detected in the cytoplasm but not the nucleus of cells. Deletions within the surrounding sequence Lys-128Lys-Lys-Arg-Lys-Val-Glu also produce cytoplasmic large-T and define a region of the protein involved in nuclear location.

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