Regulatory role of phosphatidate phosphatase in triacylglycerol synthesis of Saccharomyces cerevisiae - PubMed (original) (raw)

. 1984 Oct 24;796(1):110-7.

Regulatory role of phosphatidate phosphatase in triacylglycerol synthesis of Saccharomyces cerevisiae

K Hosaka et al. Biochim Biophys Acta. 1984.

Abstract

The regulatory mechanism of triacylglycerol synthesis in Saccharomyces cerevisiae was studied. The triacylglycerol content increased markedly during the entry of cells into the stationary growth phase, whereas the content of phospholipids remained unchanged. Pulse-labeling experiments to measure [14C]acetate incorporation into triacylglycerol revealed that the synthesis of triacylglycerol increased in the stationary growth phase. An increase in fatty acid synthesis was observed only in the later stage of the stationary growth phase and thus does not seem to be the principal causative factor for the triacylglycerol accumulation. Among various triacylglycerol-synthetic enzymes tested, the increase in the phosphatidate phosphatase (EC 3.1.3.4) activity was most closely correlated with the accumulation of triacylglycerol in the stationary phase. Our results show that phosphatidate phosphatase plays an important role in the regulation of triacylglycerol synthesis in S. cerevisiae.

PubMed Disclaimer

Similar articles

Cited by

Publication types

MeSH terms

Substances

LinkOut - more resources