Spontaneous deletions and duplications of sequences in the genome of cowpox virus - PubMed (original) (raw)

Spontaneous deletions and duplications of sequences in the genome of cowpox virus

D J Pickup et al. Proc Natl Acad Sci U S A. 1984 Nov.

Abstract

Examination of the genomes of 10 white-pock variants of cowpox virus strain Brighton red (CPV-BR) revealed that 9 of them had lost 32 to 38 kilobase pairs (kbp) from their right-hand ends and that the deleted sequences had been replaced by inverted copies of regions from 21 to 50 kbp long from the left-hand end of the genome. These variants thus possess inverted terminal repeats (ITRs) from 21 to 50 kbp long; all are longer than the ITRs of CPV-BR (10 kbp). The 10th variant is a simple deletion mutant that has lost the sequences between 32 and 12 kbp from the right-hand end of the genome. The limits of the inner ends of the observed deletions (between 32 and 38 kbp from the right-hand end of the CPV-BR genome) appear to be defined by the location of the nearest essential gene on the one hand and the location of the gene that encodes "pock redness" on the other. The genomes of the deletion/duplication white-pock variants appear to have been generated either by single crossover recombinational events between two CPV-BR genomes aligned in opposite directions or by the nonreciprocal transfer of genetic information. The sites where such recombination/transfer occurred were sequenced in four variants. In all of them, the sequences adjacent to such sites show no sequence homology or any other unusual structural feature. The analogous sites at the internal ends of the two ITRs of CPV-BR also were sequenced and also show no unusual features. It is likely that the ITRs of CPV-BR and of its white-pock variants, and probably those of other orthopox-virus genomes, arise as a result of nonhomologous recombination or by random nonreciprocal transfer of genetic information.

PubMed Disclaimer

Cited by

Poxviruses and the evolution of host range and virulence.
Haller SL, Peng C, McFadden G, Rothenburg S. Haller SL, et al. Infect Genet Evol. 2014 Jan;21:15-40. doi: 10.1016/j.meegid.2013.10.014. Epub 2013 Oct 24. Infect Genet Evol. 2014. PMID: 24161410 Free PMC article. Review.
Inflammatory responses and the generation of chemoattractant activity in cowpox virus-infected tissues.
Chua TP, Smith CE, Reith RW, Williamson JD. Chua TP, et al. Immunology. 1990 Feb;69(2):202-8. Immunology. 1990. PMID: 2155178 Free PMC article.
Amplification of the ribonucleotide reductase small subunit gene: analysis of novel joints and the mechanism of gene duplication in vaccinia virus.
Slabaugh MB, Roseman NA, Mathews CK. Slabaugh MB, et al. Nucleic Acids Res. 1989 Sep 12;17(17):7073-88. doi: 10.1093/nar/17.17.7073. Nucleic Acids Res. 1989. PMID: 2674905 Free PMC article.
Intramolecular homologous recombination in cells infected with temperature-sensitive mutants of vaccinia virus.
Merchlinsky M. Merchlinsky M. J Virol. 1989 May;63(5):2030-5. doi: 10.1128/JVI.63.5.2030-2035.1989. J Virol. 1989. PMID: 2704074 Free PMC article.
Delay of vaccinia virus-induced apoptosis in nonpermissive Chinese hamster ovary cells by the cowpox virus CHOhr and adenovirus E1B 19K genes.
Ink BS, Gilbert CS, Evan GI. Ink BS, et al. J Virol. 1995 Feb;69(2):661-8. doi: 10.1128/JVI.69.2.661-668.1995. J Virol. 1995. PMID: 7815529 Free PMC article.

References

1. Proc Natl Acad Sci U S A. 1984 Jan;81(1):206-10 - PubMed
1. J Gen Virol. 1984 May;65 ( Pt 5):875-86 - PubMed
1. Proc Natl Acad Sci U S A. 1984 Jun;81(11):3476-80 - PubMed
1. Virology. 1958 Jun;5(3):502-29 - PubMed
1. Virology. 1962 Sep;18:9-18 - PubMed

Spontaneous deletions and duplications of sequences in the genome of cowpox virus - PubMed (original) (raw)