The NDA3 gene of fission yeast encodes beta-tubulin: a cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis - PubMed (original) (raw)
The NDA3 gene of fission yeast encodes beta-tubulin: a cold-sensitive nda3 mutation reversibly blocks spindle formation and chromosome movement in mitosis
Y Hiraoka et al. Cell. 1984 Dec.
Abstract
The cells of a cold-sensitive mutant nda3-KM311 of the fission yeast Schizosaccharomyces pombe were arrested highly synchronously at a step similar to mitotic prophase when incubated at a restrictive temperature. DAPI staining and indirect immunofluorescence microscopy showed three condensed chromosomes but no spindle. Six minutes after the temperature shifted to a permissive one, the spindle appeared and elongated. The chromosomes were separated at a constant speed (relative velocity 1 micron/min), and the spindle disappeared after the chromosomes reached opposite ends of the cell. The NDA3 gene of S. pombe was cloned by transformation. The 2.6 kb Hind III genomic DNA that complemented the nda3 mutations had only one coding frame split with five short introns. The predicted amino acid sequence contained 448 residues, and was 75% homologous to that of chicken beta-tubulin.
Similar articles
- A temperature-sensitive mutation of the Schizosaccharomyces pombe gene nuc2+ that encodes a nuclear scaffold-like protein blocks spindle elongation in mitotic anaphase.
Hirano T, Hiraoka Y, Yanagida M. Hirano T, et al. J Cell Biol. 1988 Apr;106(4):1171-83. doi: 10.1083/jcb.106.4.1171. J Cell Biol. 1988. PMID: 3283148 Free PMC article. - Cold-sensitive nuclear division arrest mutants of the fission yeast Schizosaccharomyces pombe.
Toda T, Umesono K, Hirata A, Yanagida M. Toda T, et al. J Mol Biol. 1983 Aug 5;168(2):251-70. doi: 10.1016/s0022-2836(83)80017-5. J Mol Biol. 1983. PMID: 6887244 - Synchronizing Progression of Schizosaccharomyces pombe Cells from Prophase through Mitosis and into S Phase with nda3-KM311 Arrest Release.
Hagan IM, Grallert A, Simanis V. Hagan IM, et al. Cold Spring Harb Protoc. 2016 Aug 1;2016(8). doi: 10.1101/pdb.prot091256. Cold Spring Harb Protoc. 2016. PMID: 27480719
Cited by
- Negative regulation of APC/C activation by MAPK-mediated attenuation of Cdc20Slp1 under stress.
Sun L, Chen X, Song C, Shi W, Liu L, Bai S, Wang X, Chen J, Jiang C, Wang SM, Luo ZQ, Wang R, Wang Y, Jin QW. Sun L, et al. Elife. 2024 Oct 16;13:RP97896. doi: 10.7554/eLife.97896. Elife. 2024. PMID: 39412391 Free PMC article. - The fork protection complex promotes parental histone recycling and epigenetic memory.
Charlton SJ, Flury V, Kanoh Y, Genzor AV, Kollenstart L, Ao W, Brøgger P, Weisser MB, Adamus M, Alcaraz N, Delvaux de Fenffe CM, Mattiroli F, Montoya G, Masai H, Groth A, Thon G. Charlton SJ, et al. Cell. 2024 Sep 5;187(18):5029-5047.e21. doi: 10.1016/j.cell.2024.07.017. Epub 2024 Aug 1. Cell. 2024. PMID: 39094569 Free PMC article. - MAPK-dependent control of mitotic progression in S. pombe.
Iglesias-Romero AB, Soto T, Flor-Parra I, Salas-Pino S, Ruiz-Romero G, Gould KL, Cansado J, Daga RR. Iglesias-Romero AB, et al. BMC Biol. 2024 Mar 25;22(1):71. doi: 10.1186/s12915-024-01865-6. BMC Biol. 2024. PMID: 38523261 Free PMC article. - Resistance to Chemotherapeutic 5-Fluorouracil Conferred by Modulation of Heterochromatic Integrity through Ino80 Function in Fission Yeast.
Lim KK, Koh NZH, Zeng YB, Chuan JK, Raechell R, Chen ES. Lim KK, et al. Int J Mol Sci. 2023 Jun 26;24(13):10687. doi: 10.3390/ijms241310687. Int J Mol Sci. 2023. PMID: 37445861 Free PMC article. - Recovery from spindle checkpoint-mediated arrest requires a novel Dnt1-dependent APC/C activation mechanism.
Bai S, Sun L, Wang X, Wang SM, Luo ZQ, Wang Y, Jin QW. Bai S, et al. PLoS Genet. 2022 Sep 15;18(9):e1010397. doi: 10.1371/journal.pgen.1010397. eCollection 2022 Sep. PLoS Genet. 2022. PMID: 36108046 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Medical
Molecular Biology Databases