A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene - PubMed (original) (raw)

A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene

G W Stuart et al. Proc Natl Acad Sci U S A. 1984 Dec.

Abstract

To define DNA sequences involved in mouse metallothionein-I (MT-I) gene promoter function and metal regulation, we fused the 5' flanking sequences of the MT-I gene to the coding sequences of a viral thymidine kinase (TK) gene. A series of 5' deletion, 3' deletion, linker-scanning, and internal deletion mutants of the MT-I promoter was constructed and assayed by microinjection into mouse eggs. The results indicate that at least two related promoter elements can confer some metal regulation independently. Those mutations that had the most severe effect on regulation impinge on a 12-base-pair conserved sequence that is repeated several times within the mouse MT-I and other MT promoters. To test the regulatory function of this sequence, it was synthesized as a pair of complementary oligonucleotides and inserted into the promoter of the TK gene. A single insertion of this sequence conferred limited metal regulation onto the TK promoter, whereas a construct with two separate inserts was regulated as efficiently as the MT-I promoter.

PubMed Disclaimer

Cited by

Single nucleotide in the MTF-1 binding site can determine metal-specific transcription activation.
Sims HI, Chirn GW, Marr MT 2nd. Sims HI, et al. Proc Natl Acad Sci U S A. 2012 Oct 9;109(41):16516-21. doi: 10.1073/pnas.1207737109. Epub 2012 Sep 24. Proc Natl Acad Sci U S A. 2012. PMID: 23012419 Free PMC article.
The human cytomegalovirus 2.7-kilobase RNA promoter contains a functional binding site for the adenovirus major late transcription factor.
Klucher KM, Spector DH. Klucher KM, et al. J Virol. 1990 Sep;64(9):4189-98. doi: 10.1128/JVI.64.9.4189-4198.1990. J Virol. 1990. PMID: 2166813 Free PMC article.
Conditional immortalization of human thyroid epithelial cells: a tool for analysis of oncogene action.
Wynford-Thomas D, Bond JA, Wyllie FS, Burns JS, Williams ED, Jones T, Sheer D, Lemoine NR. Wynford-Thomas D, et al. Mol Cell Biol. 1990 Oct;10(10):5365-77. doi: 10.1128/mcb.10.10.5365-5377.1990. Mol Cell Biol. 1990. PMID: 1697930 Free PMC article.
A cis-acting element and associated binding factor required for CNS expression of the Drosophila melanogaster dopa decarboxylase gene.
Bray SJ, Johnson WA, Hirsh J, Heberlein U, Tjian R. Bray SJ, et al. EMBO J. 1988 Jan;7(1):177-88. doi: 10.1002/j.1460-2075.1988.tb02798.x. EMBO J. 1988. PMID: 2834202 Free PMC article.
Characterization of Gmhsp26-A, a stress gene encoding a divergent heat shock protein of soybean: heavy-metal-induced inhibition of intron processing.
Czarnecka E, Nagao RT, Key JL, Gurley WB. Czarnecka E, et al. Mol Cell Biol. 1988 Mar;8(3):1113-22. doi: 10.1128/mcb.8.3.1113-1122.1988. Mol Cell Biol. 1988. PMID: 2835661 Free PMC article.

References

1. Mol Cell Biol. 1982 Dec;2(12):1628-32 - PubMed
1. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6511-5 - PubMed
1. Eur J Biochem. 1981 Jan;113(2):267-72 - PubMed
1. J Biol Chem. 1981 Mar 25;256(6):2621-4 - PubMed
1. Nature. 1981 May 28;291(5813):340-2 - PubMed

A 12-base-pair DNA motif that is repeated several times in metallothionein gene promoters confers metal regulation to a heterologous gene - PubMed (original) (raw)