Patch-clamp study of the tetrodotoxin-resistant sodium current in group C sensory neurones - PubMed (original) (raw)
Patch-clamp study of the tetrodotoxin-resistant sodium current in group C sensory neurones
J L Bossu et al. Neurosci Lett. 1984.
Abstract
Conditions were devised to isolate in cranial sensory neurones transfer of Na ions: K and Ca were omitted from the extracellular medium, and simultaneously cells were intracellularly loaded with 120 mM caesium and 20 mM TEA at [Ca]i = 10(-8) M. A tetrodotoxin (TTX)-resistant current was shown to be elicited by step depolarization from -25 MV upwards. This current successively activates and inactivates at increasing rates on further depolarization and at 0 mV (where peak amplitude is reached) its time course is of 20-50 ms. Absence of TTX-sensitivity (up to 15 microM), slow time course and an activation curve shifted by 15 mV towards the depolarized potentials differentiate this current from the more classical fast Na current which can be elicited on the same cells. Inactivation was provoked by a prepulse of varying amplitude and duration: with a prepulse command to -20 mV, inactivation was of 50% within a delay of 300 ms and almost 100% in about 1 min. After complete inactivation by command to 0 mV for 300 ms, recovery by holding the potential at -80 mV was of 50% in 205 ms, and of 100% after 1-4 s. It is concluded that a charge transfer of Na accounts for most of the hump which prolongs the action potential of these sensory neurones, and thus it can be proposed that spike duration as modulated by neurotransmitters may also involve Na in addition to Ca.
Similar articles
- Na+ and Ca2+ currents of acutely isolated adult rat nodose ganglion cells.
Ikeda SR, Schofield GG, Weight FF. Ikeda SR, et al. J Neurophysiol. 1986 Mar;55(3):527-39. doi: 10.1152/jn.1986.55.3.527. J Neurophysiol. 1986. PMID: 2420945 - Voltage- and use-dependent inhibition of Na+ channels in rat sensory neurones by 4030W92, a new antihyperalgesic agent.
Trezise DJ, John VH, Xie XM. Trezise DJ, et al. Br J Pharmacol. 1998 Jul;124(5):953-63. doi: 10.1038/sj.bjp.0701919. Br J Pharmacol. 1998. PMID: 9692781 Free PMC article. - Characterization of voltage-sensitive Na+ and K+ currents recorded from acutely dissociated pelvic ganglion neurons of the adult rat.
Yoshimura N, De Groat WC. Yoshimura N, et al. J Neurophysiol. 1996 Oct;76(4):2508-21. doi: 10.1152/jn.1996.76.4.2508. J Neurophysiol. 1996. PMID: 8899623 - Voltage-dependent sodium and calcium currents in cultured parasympathetic neurones from rat intracardiac ganglia.
Xu ZJ, Adams DJ. Xu ZJ, et al. J Physiol. 1992 Oct;456:425-41. doi: 10.1113/jphysiol.1992.sp019344. J Physiol. 1992. PMID: 1338101 Free PMC article. - Voltage-dependent conductances of solitary ganglion cells dissociated from the rat retina.
Lipton SA, Tauck DL. Lipton SA, et al. J Physiol. 1987 Apr;385:361-91. doi: 10.1113/jphysiol.1987.sp016497. J Physiol. 1987. PMID: 2443669 Free PMC article. Review.
Cited by
- A review of dorsal root ganglia and primary sensory neuron plasticity mediating inflammatory and chronic neuropathic pain.
Jang K, Garraway SM. Jang K, et al. Neurobiol Pain. 2024 Jan 20;15:100151. doi: 10.1016/j.ynpai.2024.100151. eCollection 2024 Jan-Jun. Neurobiol Pain. 2024. PMID: 38314104 Free PMC article. Review. - Human microbiota research in Africa: a systematic review reveals gaps and priorities for future research.
Allali I, Abotsi RE, Tow LA, Thabane L, Zar HJ, Mulder NM, Nicol MP. Allali I, et al. Microbiome. 2021 Dec 15;9(1):241. doi: 10.1186/s40168-021-01195-7. Microbiome. 2021. PMID: 34911583 Free PMC article. Review. - Tetrodotoxin-insensitive electrical field stimulation-induced contractions on Crotalus durissus terrificus corpus cavernosum.
Campos R, Mónica FZ, Rodrigues RL, Rojas-Moscoso JA, Moreno RA, Cogo JC, de Oliveira MA, Antunes E, De Nucci G. Campos R, et al. PLoS One. 2017 Aug 24;12(8):e0183766. doi: 10.1371/journal.pone.0183766. eCollection 2017. PLoS One. 2017. PMID: 28837636 Free PMC article. - Characteristics of sodium currents in rat geniculate ganglion neurons.
Nakamura S, Bradley RM. Nakamura S, et al. J Neurophysiol. 2011 Dec;106(6):2982-91. doi: 10.1152/jn.00369.2011. Epub 2011 Sep 14. J Neurophysiol. 2011. PMID: 21917997 Free PMC article. - Kv1.3 channels regulate synaptic transmission in the nucleus of solitary tract.
Ramirez-Navarro A, Glazebrook PA, Kane-Sutton M, Padro C, Kline DD, Kunze DL. Ramirez-Navarro A, et al. J Neurophysiol. 2011 Jun;105(6):2772-80. doi: 10.1152/jn.00494.2010. Epub 2011 Mar 23. J Neurophysiol. 2011. PMID: 21430270 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources