Measurement of receptor induced changes in intracellular free calcium in human platelets - PubMed (original) (raw)
Measurement of receptor induced changes in intracellular free calcium in human platelets
P Erne et al. J Recept Res. 1984.
Abstract
The intracellular free calcium concentration plays a key role as second messenger in the regulation of cellular reactions. Post-receptor events can be investigated by measurement of free calcium concentration in cells. Our experience in measuring the intracellular free calcium concentration in platelets with the use of the fluorescent indicator quin-2-tetraacetoxymethyl- ester is described. Possible pitfalls in the preparation procedures of the platelets are discussed as well as critical steps and the limitations of the quin-2-method. The methodological approach is demonstrated by the presentation of an investigation with the adenylate cyclase inhibitors adenosine-5'-diphosphate and epinephrine as well as their interrelationship. The potentiation effect of epinephrine to adenosine 5'-diphosphate on platelet function such as aggregation is accompanied by a potentiation of the effect of these two platelet activators in elevating the intracellular free calcium concentration. Adenosine 5'-diphosphate elevates intra-platelet free calcium alone, whereas epinephrine acting through stimulation of the alpha2-receptor needs another permissive factor to immediately elevate the intra-platelet free calcium.
Similar articles
- Ca+2 mobilization and fibrinogen binding of platelets refractory to adenosine diphosphate stimulation.
Peerschke EI. Peerschke EI. J Lab Clin Med. 1985 Aug;106(2):111-22. J Lab Clin Med. 1985. PMID: 2991399 - Synergistic potentiation by epinephrine of collagen or thrombin-induced calcium mobilization in human platelets.
Ardlie NG, Bell LK, McGuiness JA. Ardlie NG, et al. Thromb Res. 1987 May 15;46(4):519-26. doi: 10.1016/0049-3848(87)90153-8. Thromb Res. 1987. PMID: 3617010 - Role of cyclic AMP in adenosine inhibition of intracellular calcium rise in human platelets. Comparison of adenosine effects on thrombin- and epinephrine-induced platelet stimulation.
Feoktistov IA, Paul S, Hollister AS, Robertson D, Biaggioni I. Feoktistov IA, et al. Am J Hypertens. 1992 Jun;5(6 Pt 2):147S-153S. doi: 10.1093/ajh/5.6.147s. Am J Hypertens. 1992. PMID: 1321639 - Removal of extraplatelet Na+ eliminates indomethacin-sensitive secretion from human platelets stimulated by epinephrine, ADP, and thrombin.
Connolly TM, Limbird LE. Connolly TM, et al. Proc Natl Acad Sci U S A. 1983 Sep;80(17):5320-4. doi: 10.1073/pnas.80.17.5320. Proc Natl Acad Sci U S A. 1983. PMID: 6577431 Free PMC article.
Cited by
- Effects of mibefradil on intracellular Ca2+ release in cultured rat cardiac fibroblasts and human platelets.
Eberhard M, Miyagawa K, Hermsmeyer K, Erne P. Eberhard M, et al. Naunyn Schmiedebergs Arch Pharmacol. 1995 Dec;353(1):94-101. doi: 10.1007/BF00168921. Naunyn Schmiedebergs Arch Pharmacol. 1995. PMID: 8750922 - Blood platelets in human essential hypertension.
De Clerck F. De Clerck F. Agents Actions. 1986 Aug;18(5-6):563-80. doi: 10.1007/BF01964966. Agents Actions. 1986. PMID: 3532721 Review. - Altered protein kinase C in a mast cell variant defective in exocytosis.
Mazurek N, Regazzi R, Borner C, Wyss R, Conscience JF, Erne P, Eppenberger U, Fabbro D. Mazurek N, et al. Proc Natl Acad Sci U S A. 1987 Mar;84(5):1277-81. doi: 10.1073/pnas.84.5.1277. Proc Natl Acad Sci U S A. 1987. PMID: 3493490 Free PMC article. - Vasopressin-induced activation of human blood platelets: prominent role of Mg2+.
Erne P, Pletscher A. Erne P, et al. Naunyn Schmiedebergs Arch Pharmacol. 1985 Mar;329(1):97-9. doi: 10.1007/BF00695199. Naunyn Schmiedebergs Arch Pharmacol. 1985. PMID: 3158825 - Low density lipoprotein causes general cellular activation with increased phosphatidylinositol turnover and lipoprotein catabolism.
Block LH, Knorr M, Vogt E, Locher R, Vetter W, Groscurth P, Qiao BY, Pometta D, James R, Regenass M, et al. Block LH, et al. Proc Natl Acad Sci U S A. 1988 Feb;85(3):885-9. doi: 10.1073/pnas.85.3.885. Proc Natl Acad Sci U S A. 1988. PMID: 2829208 Free PMC article.