Gonococcal pilus subunit size heterogeneity correlates with transitions in colony piliation phenotype, not with changes in colony opacity - PubMed (original) (raw)
Gonococcal pilus subunit size heterogeneity correlates with transitions in colony piliation phenotype, not with changes in colony opacity
J Swanson et al. J Exp Med. 1983.
Abstract
The apparent subunit sizes for pili of gonococci (Gc) have been visualized by using either Iodogen 125I-labeled whole Gc or immunoblotting with antipilus antiserum. These methods permitted definition of pilus subunit sizes for Gc of a given strain that had undergone changes either in piliation phenotype or in colonial opacity/protein II phenotype. The results indicate that pilus subunit size does not change coincident with changes in colony opacity/protein II phenotypes; but change in pilus subunit size is seen after a change in piliation phenotype (P+ leads to P++, and vice versa). Marked diversity in pilus subunit sizes is found for Gc of individual strains when P+ derivatives of P- colonies are compared. This diversity extends to pilus subunits of Gc found in single colonies; two distinct pilus forms were demonstrated for Gc residing in several single colonies. These findings show that Gc of a given strain are able to express any of a number of different pilus subunit size forms.
Similar articles
- Pilus- gonococcal variants. Evidence for multiple forms of piliation control.
Swanson J, Bergström S, Barrera O, Robbins K, Corwin D. Swanson J, et al. J Exp Med. 1985 Aug 1;162(2):729-44. doi: 10.1084/jem.162.2.729. J Exp Med. 1985. PMID: 2410533 Free PMC article. - Gene conversion accounts for pilin structural changes and for reversible piliation "phase" changes in gonococci.
Swanson J, Bergstrom S, Boslego J, Koomey M. Swanson J, et al. Antonie Van Leeuwenhoek. 1987;53(6):441-6. doi: 10.1007/BF00415500. Antonie Van Leeuwenhoek. 1987. PMID: 2897187 - Piliation control mechanisms in Neisseria gonorrhoeae.
Bergström S, Robbins K, Koomey JM, Swanson J. Bergström S, et al. Proc Natl Acad Sci U S A. 1986 Jun;83(11):3890-4. doi: 10.1073/pnas.83.11.3890. Proc Natl Acad Sci U S A. 1986. PMID: 2872674 Free PMC article. - Intra-strain heterogeneity of gonococcal pili is related to opacity colony variance.
Salit IE, Blake M, Gotschlich EC. Salit IE, et al. J Exp Med. 1980 Mar 1;151(3):716-25. doi: 10.1084/jem.151.3.716. J Exp Med. 1980. PMID: 6102108 Free PMC article. - Chaperone-assisted assembly and molecular architecture of adhesive pili.
Hultgren SJ, Normark S, Abraham SN. Hultgren SJ, et al. Annu Rev Microbiol. 1991;45:383-415. doi: 10.1146/annurev.mi.45.100191.002123. Annu Rev Microbiol. 1991. PMID: 1683764 Review.
Cited by
- Phase variable colony variants are conserved across Gardnerella spp. and exhibit different virulence-associated phenotypes.
Garcia EM, Klimowicz AK, Edupuganti L, Topf MA, Bhide SR, Slusser DJ, Leib SM, Coddington CL, Matveyev A, Buck GA, Jefferson KK, Pepperell CS, Dillard JP. Garcia EM, et al. mSphere. 2024 Jul 30;9(7):e0045024. doi: 10.1128/msphere.00450-24. Epub 2024 Jun 27. mSphere. 2024. PMID: 38926904 Free PMC article. - Distinct Patterns of Host Adherence by Neisseria gonorrhoeae Isolated from Experimental Gonorrhea.
He Y, Zhang S, Zhang Y, Wu B, Xue Y, Ye C, Li Q, Olivia AN, Tembo JM, Chen H, Cai H, Chen T. He Y, et al. Can J Infect Dis Med Microbiol. 2021 May 13;2021:7865405. doi: 10.1155/2021/7865405. eCollection 2021. Can J Infect Dis Med Microbiol. 2021. PMID: 34093925 Free PMC article. - Isolation and characterization of Neisseria musculi sp. nov., from the wild house mouse.
Weyand NJ, Ma M, Phifer-Rixey M, Taku NA, Rendón MA, Hockenberry AM, Kim WJ, Agellon AB, Biais N, Suzuki TA, Goodyer-Sait L, Harrison OB, Bratcher HB, Nachman MW, Maiden MCJ, So M. Weyand NJ, et al. Int J Syst Evol Microbiol. 2016 Sep;66(9):3585-3593. doi: 10.1099/ijsem.0.001237. Epub 2016 Jun 13. Int J Syst Evol Microbiol. 2016. PMID: 27298306 Free PMC article. - H-NS suppresses pilE intragenic transcription and antigenic variation in Neisseria gonorrhoeae.
Masters TL, Wachter S, Wachter J, Hill SA. Masters TL, et al. Microbiology (Reading). 2016 Jan;162(1):177-190. doi: 10.1099/mic.0.000199. Epub 2015 Oct 16. Microbiology (Reading). 2016. PMID: 26475082 Free PMC article. - Implications of phase variation of a gene (pgtA) encoding a pilin galactosyl transferase in gonococcal pathogenesis.
Banerjee A, Wang R, Supernavage SL, Ghosh SK, Parker J, Ganesh NF, Wang PG, Gulati S, Rice PA. Banerjee A, et al. J Exp Med. 2002 Jul 15;196(2):147-62. doi: 10.1084/jem.20012022. J Exp Med. 2002. PMID: 12119340 Free PMC article.
References
- J Exp Med. 1975 Jun 1;141(6):1470-5 - PubMed
- Cell. 1982 Aug;30(1):45-52 - PubMed
- Infect Immun. 1976 Aug;14(2):593-5 - PubMed
- J Infect Dis. 1976 Oct;134(4):377-83 - PubMed
- J Gen Microbiol. 1977 Sep;102(1):169-77 - PubMed
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Miscellaneous