A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters - PubMed (original) (raw)
- PMID: 6143576
- DOI: 10.1007/BF01120313
A rapid and efficient method to enrich SAF-protein from scrapie brains of hamsters
H Hilmert et al. Biosci Rep. 1984 Feb.
Abstract
Scrapie hamster brains contain at least 5-10 micrograms of scrapie-associated fibrils (SAF) per brain as estimated by the amount of its major constituent, a protein of about 26 000 daltons (SAF-protein). It can be extracted efficiently by a 10% solution of sarkosyl and can be enriched by differential centrifugation and buffer extraction. Scrapie infectivity, SAF, and SAF-protein copurify.
Similar articles
- The major protein of SAF is absent from spleen and thus not an essential part of the scrapie agent.
Czub M, Braig HR, Blode H, Diringer H. Czub M, et al. Arch Virol. 1986;91(3-4):383-6. doi: 10.1007/BF01314297. Arch Virol. 1986. PMID: 2877648 - Structural and biochemical evidence that scrapie-associated fibrils assemble in vivo.
Somerville RA, Ritchie LA, Gibson PH. Somerville RA, et al. J Gen Virol. 1989 Jan;70 ( Pt 1):25-35. doi: 10.1099/0022-1317-70-1-25. J Gen Virol. 1989. PMID: 2567338 - Scrapie-infected spleens: analysis of infectivity, scrapie-associated fibrils, and protease-resistant proteins.
Rubenstein R, Merz PA, Kascsak RJ, Scalici CL, Papini MC, Carp RI, Kimberlin RH. Rubenstein R, et al. J Infect Dis. 1991 Jul;164(1):29-35. doi: 10.1093/infdis/164.1.29. J Infect Dis. 1991. PMID: 1676044 - Scrapie-associated fibrils, PrP protein and the Sinc gene.
Hope J, Hunter N. Hope J, et al. Ciba Found Symp. 1988;135:146-63. doi: 10.1002/9780470513613.ch10. Ciba Found Symp. 1988. PMID: 2900718 Review. - The nature of the unconventional slow infection agents remains a puzzle.
Carp RI, Kascsak RJ, Wisniewski HM, Merz PA, Rubenstein R, Bendheim P, Bolton D. Carp RI, et al. Alzheimer Dis Assoc Disord. 1989 Spring-Summer;3(1-2):79-99. doi: 10.1097/00002093-198903010-00008. Alzheimer Dis Assoc Disord. 1989. PMID: 2568119 Review.
Cited by
- Exploring the cause of initially reactive bovine brains on rapid tests for BSE.
Dudas S, James J, Anderson R, Czub S. Dudas S, et al. Prion. 2015;9(6):429-43. doi: 10.1080/19336896.2015.1115945. Prion. 2015. PMID: 26689488 Free PMC article. - Highly infectious CJD particles lack prion protein but contain many viral-linked peptides by LC-MS/MS.
Kipkorir T, Tittman S, Botsios S, Manuelidis L. Kipkorir T, et al. J Cell Biochem. 2014 Nov;115(11):2012-21. doi: 10.1002/jcb.24873. J Cell Biochem. 2014. PMID: 24933657 Free PMC article. - A comparative study of modified confirmatory techniques and additional immuno-based methods for non-conclusive autolytic bovine spongiform encephalopathy cases.
Sarasa R, Becher D, Badiola JJ, Monzón M. Sarasa R, et al. BMC Vet Res. 2013 Oct 18;9:212. doi: 10.1186/1746-6148-9-212. BMC Vet Res. 2013. PMID: 24138967 Free PMC article. - Influence of Mabs on PrP(Sc) formation using in vitro and cell-free systems.
Chang B, Petersen R, Wisniewski T, Rubenstein R. Chang B, et al. PLoS One. 2012;7(7):e41626. doi: 10.1371/journal.pone.0041626. Epub 2012 Jul 27. PLoS One. 2012. PMID: 22848548 Free PMC article. - Comparative profiling of highly enriched 22L and Chandler mouse scrapie prion protein preparations.
Moore RA, Timmes A, Wilmarth PA, Priola SA. Moore RA, et al. Proteomics. 2010 Aug;10(15):2858-69. doi: 10.1002/pmic.201000104. Proteomics. 2010. PMID: 20518029 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources