Identification of a protein kinase as an intrinsic component of rat liver coated vesicles - PubMed (original) (raw)
Identification of a protein kinase as an intrinsic component of rat liver coated vesicles
C Campbell et al. Biochemistry. 1984.
Abstract
Purified rat liver coated vesicles phosphorylate two peptides, Mr 53 000 and Mr 51 000, in the presence of [gamma-32P]ATP. Incorporation of phosphate into these peptides is not stimulated by cAMP, Ca2+, or Ca2+ plus calmodulin and occurs principally on a threonine residue. Mild conditions that result in removal of coat proteins from coated vesicles remove most of the protein kinase activity, suggesting the enzyme(s) is (are) not an integral membrane protein. Photolabeling of coated vesicles with 8-azido-[alpha-32P]ATP results in specific labeling of only the Mr 53 000 and Mr 51 000 peptides. Preincubation with 10 mM N-ethylmaleimide inhibits kinase activity and concomitantly reduces photolabeling of the two peptides. Thus, the data are consistent with the hypothesis that protein kinase activity resides with these two coated vesicle proteins and that they are catalyzing an autophosphorylation reaction.
Similar articles
- Protein kinase and its endogenous substrates in coated vesicles.
Usami M, Takahashi A, Kadota K. Usami M, et al. Biochim Biophys Acta. 1984 Apr 24;798(3):306-12. doi: 10.1016/0304-4165(84)90103-x. Biochim Biophys Acta. 1984. PMID: 6143572 - Protein kinase(s) in bovine brain coated vesicles.
Pauloin A, Loeb J, Jollés P. Pauloin A, et al. Biochim Biophys Acta. 1984 Jun 29;799(3):238-45. doi: 10.1016/0304-4165(84)90266-6. Biochim Biophys Acta. 1984. PMID: 6145448 - Effects of Ca2+, calmodulin and cyclic AMP on the phosphorylation of endogenous proteins by homogenates of rt islets of langerhans.
Harrison DE, Ashcroft SJ. Harrison DE, et al. Biochim Biophys Acta. 1982 Feb 2;714(2):313-9. doi: 10.1016/0304-4165(82)90339-7. Biochim Biophys Acta. 1982. PMID: 6275912 - Phosphorylation of brain synaptic and coated vesicle proteins by endogenous Ca2+/calmodulin- and cAMP-dependent protein kinases.
Moskowitz N, Glassman A, Ores C, Schook W, Puszkin S. Moskowitz N, et al. J Neurochem. 1983 Mar;40(3):711-8. doi: 10.1111/j.1471-4159.1983.tb08037.x. J Neurochem. 1983. PMID: 6298368
Cited by
- Receptors compete for adaptors found in plasma membrane coated pits.
Pearse BM. Pearse BM. EMBO J. 1988 Nov;7(11):3331-6. doi: 10.1002/j.1460-2075.1988.tb03204.x. EMBO J. 1988. PMID: 2905261 Free PMC article. - Structural relationships between clathrin assembly proteins from the Golgi and the plasma membrane.
Ahle S, Mann A, Eichelsbacher U, Ungewickell E. Ahle S, et al. EMBO J. 1988 Apr;7(4):919-29. doi: 10.1002/j.1460-2075.1988.tb02897.x. EMBO J. 1988. PMID: 3402440 Free PMC article. - Coupling between clathrin-dependent endocytic budding and F-BAR-dependent tubulation in a cell-free system.
Wu M, Huang B, Graham M, Raimondi A, Heuser JE, Zhuang X, De Camilli P. Wu M, et al. Nat Cell Biol. 2010 Sep;12(9):902-8. doi: 10.1038/ncb2094. Epub 2010 Aug 22. Nat Cell Biol. 2010. PMID: 20729836 Free PMC article. - The autosomal recessive hypercholesterolemia (ARH) protein interfaces directly with the clathrin-coat machinery.
Mishra SK, Watkins SC, Traub LM. Mishra SK, et al. Proc Natl Acad Sci U S A. 2002 Dec 10;99(25):16099-104. doi: 10.1073/pnas.252630799. Epub 2002 Nov 25. Proc Natl Acad Sci U S A. 2002. PMID: 12451172 Free PMC article. - Simian and human immunodeficiency virus Nef proteins use different surfaces to downregulate class I major histocompatibility complex antigen expression.
Swigut T, Iafrate AJ, Muench J, Kirchhoff F, Skowronski J. Swigut T, et al. J Virol. 2000 Jun;74(12):5691-701. doi: 10.1128/jvi.74.12.5691-5701.2000. J Virol. 2000. PMID: 10823877 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Miscellaneous