Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin - PubMed (original) (raw)

PMID: 6153393
PMCID: PMC371419
DOI: 10.1172/JCI109723

Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin

D A Ausiello et al. J Clin Invest. 1980 Mar.

Abstract

Studies to identify the physiological role of glomerular mesangial cells were undertaken using homogeneous cultures of rat glomerular cells of apparent mesangial origin (MS). Cultured MS cells were treated with arginine vasopressin (AVP), angiotensin II (AGII), prostaglandin E(2), and parathyroid hormone. AVP (0.1 nM) and AGII (1 nM) stimulated contraction of MS cells in vitro that was complete by 2 min at 37 degrees C or 10 min at 23 degrees C as observed by phase contrast and electron microscopy. Relaxation recurred 15 min after hormonal addition at 23 degrees C. Similar experiments in cloned rat glomerular epithelial cells or "renin"-producing cells did not demonstrate a contractile response. The contraction of MS cells was independent of cyclic AMP (cAMP) and cyclic 3',5'-guanosine monophosphate (cGMP) production, even when cyclic nucleotides were measured as early as 30 s after hormonal stimulation. To demonstrate that contraction was a function of hormone-receptor interaction, binding of [(3)H](8-lysine)vasopressin was studied. Specific binding for 1.6 and 5 nM hormone was both time- and dose-dependent. The estimated apparent affinity was 10 nM. In late MS cell passages (>16th) that no longer demonstrated hormone-stimulated contraction, no specific binding of [(3)H](8-lysine)vasopressin was observed. Incubations were modified to optimize the conditions for detecting the effect of hormones on cell cyclic nucleotide content. A supramaximal concentration of AVP (200 nM) increased the cAMP content of MS cells twofold in the presence of a phosphodiesterase inhibitor. Similar experiments with prostaglandin E(2) (1 mug/ml) led to a 1.5-6-fold increase in MS cell cAMP content, but no effect on contraction was observed. Neither hormone altered cGMP content. These data are further support for the independence of contraction and cyclic nucleotide production. Our studies suggest that MS cells are the equivalent of smooth muscle cells in the glomerulus and that their contraction may be important in control of glomerular filtration.

PubMed Disclaimer

Cited by

The Glomerulus According to the Mesangium.
Ebefors K, Bergwall L, Nyström J. Ebefors K, et al. Front Med (Lausanne). 2022 Jan 26;8:740527. doi: 10.3389/fmed.2021.740527. eCollection 2021. Front Med (Lausanne). 2022. PMID: 35155460 Free PMC article. Review.
A Novel Interaction between FLICE-Associated Huge Protein (FLASH) and E2A Regulates Cell Proliferation and Cellular Senescence via Tumor Necrosis Factor (TNF)-Alpha-p21WAF1/CIP1 Axis.
Hirano T, Murakami T, Ono H, Sakurai A, Tominaga T, Takahashi T, Nagai K, Doi T, Abe H. Hirano T, et al. PLoS One. 2015 Jul 24;10(7):e0133205. doi: 10.1371/journal.pone.0133205. eCollection 2015. PLoS One. 2015. PMID: 26208142 Free PMC article.
Simultaneous registration of Ca(2+) transients and volume changes in rat mesangial cells: Evaluation of the effects of protein kinase C down-regulation.
Ochsner M, Fleck T, Kernen P, Deranleau DA, Pfeilschifter J. Ochsner M, et al. J Fluoresc. 1992 Mar;2(1):37-45. doi: 10.1007/BF00866387. J Fluoresc. 1992. PMID: 24243157
Real-time Imaging of Ca-handling in Intact Renal Glomeruli Using Confocal Microscopy.
Ghayur MN, Janssen LJ. Ghayur MN, et al. Med Hypotheses Res. 2009 Jul;5(1/2):47-56. Med Hypotheses Res. 2009. PMID: 22287941 Free PMC article.
CONTRACTILITY OF THE RENAL GLOMERULUS AND MESANGIAL CELLS: LINGERING DOUBTS AND STRATEGIES FOR THE FUTURE.
Ghayur MN, Krepinsky JC, Janssen LJ. Ghayur MN, et al. Med Hypotheses Res. 2008 Jan;4(1):1-9. Med Hypotheses Res. 2008. PMID: 22287940 Free PMC article.

References

1. Nephron. 1969;6(1):1-10 - PubMed
1. J Cell Biol. 1969 Sep;42(3):647-72 - PubMed
1. Am J Physiol. 1970 Jul;219(1):222-9 - PubMed
1. N Engl J Med. 1971 Mar 4;284(9):482-90 - PubMed
1. Am J Pathol. 1972 Jan;66(1):97-110 - PubMed

Contraction of cultured rat glomerular cells of apparent mesangial origin after stimulation with angiotensin II and arginine vasopressin - PubMed (original) (raw)