Release of somatomedin-like activity by cultured WI-38 human fibroblasts - PubMed (original) (raw)

Release of somatomedin-like activity by cultured WI-38 human fibroblasts

P R Atkison et al. Endocrinology. 1980 Jun.

Abstract

Confluent cultures of normal diploid WI-38 human embryonic lung fibroblasts released somatomedin (SM)-like activity into their incubation medium during culture in serum-free medium. This postculture medium (conditioned medium) stimulated cell division in these same cultured WI-38 fibroblasts and 35SO4 uptake by hypophysectomized rat cartilage in vitro. The conditioned medium also contained immunoreactive SM (IRSM) activity which yielded parallel dose-response curves to human serum in a RIA for SM. The IRSM activity measured in conditioned medium was not the artifactual result of effects of possible SM-binding proteins or proteolytic enzymes in conditioned medium. These studies suggest that cultured WI-38 fibroblasts produce and release SM-like activity which has SM-like biological activity and is immunoreactive with a basic SM purified from human plasma Cohn fraction and having similarity with SM-C and insulin-like growth factor-I. Human GH appears to stimulate production and release of IRSM activity by these cells.

PubMed Disclaimer

Similar articles

• Partial characterization of a mitogenic factor with somatomedin-like activity produced by culture WI-38 human fibroblasts.
  Atkison PR, Bala RM. Atkison PR, et al. J Cell Physiol. 1981 Jun;107(3):317-27. doi: 10.1002/jcp.1041070303. J Cell Physiol. 1981. PMID: 6972946
• Radioimmunoassay of a basic somatomedin: comparison of various assay techniques and somatomedin levels in various sera.
  Bala RM, Bhaumick B. Bala RM, et al. J Clin Endocrinol Metab. 1979 Nov;49(5):770-7. doi: 10.1210/jcem-49-5-770. J Clin Endocrinol Metab. 1979. PMID: 39945
• Embryonic chick cartilage produces its own somatomedin-like peptide to stimulate cartilage growth in vitro.
  Burch WM, Weir S, Van Wyk JJ. Burch WM, et al. Endocrinology. 1986 Sep;119(3):1370-6. doi: 10.1210/endo-119-3-1370. Endocrinology. 1986. PMID: 3732171
• Evidence for autocrine mitogenic stimulation by somatomedin-C/insulin-like growth factor I on an established human lung cancer cell line.
  Minuto F, Del Monte P, Barreca A, Alama A, Cariola G, Giordano G. Minuto F, et al. Cancer Res. 1988 Jul 1;48(13):3716-9. Cancer Res. 1988. PMID: 3378214
• Isolation and chemistry of human somatomedins A and B.
  Sievertsson H, Fryklund L, Uthne K, Hall K, Westermark B. Sievertsson H, et al. Adv Metab Disord. 1975;8:47-60. doi: 10.1016/b978-0-12-027308-9.50009-3. Adv Metab Disord. 1975. PMID: 1106159 Review. No abstract available.

Cited by

• IPF pathogenesis is dependent upon TGFβ induction of IGF-1.
  Hernandez DM, Kang JH, Choudhury M, Andrianifahanana M, Yin X, Limper AH, Leof EB. Hernandez DM, et al. FASEB J. 2020 Apr;34(4):5363-5388. doi: 10.1096/fj.201901719RR. Epub 2020 Feb 17. FASEB J. 2020. PMID: 32067272 Free PMC article.
• Roles of Proteoglycans and Glycosaminoglycans in Wound Healing and Fibrosis.
  Ghatak S, Maytin EV, Mack JA, Hascall VC, Atanelishvili I, Moreno Rodriguez R, Markwald RR, Misra S. Ghatak S, et al. Int J Cell Biol. 2015;2015:834893. doi: 10.1155/2015/834893. Epub 2015 Sep 10. Int J Cell Biol. 2015. PMID: 26448760 Free PMC article. Review.
• Endocrine parameters and phenotypes of the growth hormone receptor gene disrupted (GHR-/-) mouse.
  List EO, Sackmann-Sala L, Berryman DE, Funk K, Kelder B, Gosney ES, Okada S, Ding J, Cruz-Topete D, Kopchick JJ. List EO, et al. Endocr Rev. 2011 Jun;32(3):356-86. doi: 10.1210/er.2010-0009. Epub 2010 Dec 1. Endocr Rev. 2011. PMID: 21123740 Free PMC article. Review.
• Resistance exercise overtraining and overreaching. Neuroendocrine responses.
  Fry AC, Kraemer WJ. Fry AC, et al. Sports Med. 1997 Feb;23(2):106-29. doi: 10.2165/00007256-199723020-00004. Sports Med. 1997. PMID: 9068095 Review.
• Tissue concentrations of somatomedin C: further evidence for multiple sites of synthesis and paracrine or autocrine mechanisms of action.
  D'Ercole AJ, Stiles AD, Underwood LE. D'Ercole AJ, et al. Proc Natl Acad Sci U S A. 1984 Feb;81(3):935-9. doi: 10.1073/pnas.81.3.935. Proc Natl Acad Sci U S A. 1984. PMID: 6583688 Free PMC article.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Silverchair Information Systems