Biosynthesis of hepatitis B virus surface antigen in Escherichia coli - PubMed (original) (raw)
. 1980 Aug 28;286(5776):893-5.
doi: 10.1038/286893a0.
- PMID: 6157992
- DOI: 10.1038/286893a0
Biosynthesis of hepatitis B virus surface antigen in Escherichia coli
P Charnay et al. Nature. 1980.
Abstract
Hepatitis B is a widespread viral disease. In the absence of cell cultures capable of propagating the virus (HBV) an efficient vaccine has been prepared from viral envelopes isolated from the plasma of chronic carriers. The major polypeptide of the envelope is one of molecular weight 25,000 which carries the surface antigen (HBsAg). Therefore, the biosynthesis of this polypeptide in Escherichia coli may offer an alternative procedure to produce HbsAg free from human proteins. Recently, the HBV genome has been cloned in E.coli. Determination of its primary structure allowed the localization of the gene (called gene S) coding for HBsAg and the synthesis of the core antigen in E.coli has been reported. We have constructed a derivative of bacteriophage lambda carrying a fusion between the beta-galactosidase gene (lacZ) and the HBsAg coding sequence (lambdalacHBs-1). Infection of E.coli with lambdalacHBs-1 leads to the biosynthesis of a polypeptide of molecular weitht 138,000 carrying antigenic determinants of HBV surface antigen.
Similar articles
- Structure and expression of the hepatitis B virus genome.
Wain-Hobson S, Pourcel C, Brechot C, Charnay P, Dubois MF, Fritsch A, Gervais M, Louise A, Tiollais P. Wain-Hobson S, et al. Dev Biol Stand. 1981;50:293-300. Dev Biol Stand. 1981. PMID: 6281110 - [Synthesis and expression of the DNA fragment coding the antigenic determinant of the surface antigen protein of hepatitis B virus].
Smirnov VD, Sergienko OV, Skripkin EA, Dubichev AG, Lunin VG. Smirnov VD, et al. Bioorg Khim. 1983 Oct;9(10):1388-94. Bioorg Khim. 1983. PMID: 6207838 Russian. - Expression of multivalent pre-S1 antigen of hepatitis B virus in Escherichia coli (synthetic oligodeoxynucleotides, surface antigens, recombinant DNA, fusion protein, beta-galactosidase).
Sidorkiewicz M, Jaworski J, Płucienniczak G, Płucienniczak A, Stpień P, Kalinowska B, Karwowska S, Nowosławski A, Uznański B, Wilk A, et al. Sidorkiewicz M, et al. Arch Immunol Ther Exp (Warsz). 1991;39(4):357-64. Arch Immunol Ther Exp (Warsz). 1991. PMID: 1821612 - Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.
Pourcel C, Louise A, Gervais M, Chenciner N, Dubois MF, Tiollais P. Pourcel C, et al. J Virol. 1982 Apr;42(1):100-5. doi: 10.1128/JVI.42.1.100-105.1982. J Virol. 1982. PMID: 7086959 Free PMC article. - [Structure and expression of human hepatitis B virus surface antigen].
Kozlovskaia TM, Pumpen PP. Kozlovskaia TM, et al. Mol Biol (Mosk). 1986 Jul-Aug;20(4):884-901. Mol Biol (Mosk). 1986. PMID: 2429171 Review. Russian.
Cited by
- Beasley's 1981 paper: The power of a well-designed cohort study to drive liver cancer research and prevention.
Koshiol J, Liu Z, O'Brien TR, Hildesheim A. Koshiol J, et al. Cancer Epidemiol. 2018 Apr;53:195-199. doi: 10.1016/j.canep.2018.01.007. Epub 2018 Feb 13. Cancer Epidemiol. 2018. PMID: 29396075 Free PMC article. Review. - Effects of mRNA secondary structure on the expression of HEV ORF2 proteins in Escherichia coli.
Behloul N, Wei W, Baha S, Liu Z, Wen J, Meng J. Behloul N, et al. Microb Cell Fact. 2017 Nov 14;16(1):200. doi: 10.1186/s12934-017-0812-8. Microb Cell Fact. 2017. PMID: 29137642 Free PMC article. - Biotechnology and the transformation of vaccine innovation: The case of the hepatitis B vaccines 1968-2000.
Huzair F, Sturdy S. Huzair F, et al. Stud Hist Philos Biol Biomed Sci. 2017 Aug;64:11-21. doi: 10.1016/j.shpsc.2017.05.004. Epub 2017 May 13. Stud Hist Philos Biol Biomed Sci. 2017. PMID: 28511068 Free PMC article. - Immunogenicity of Leishmania-derived hepatitis B small surface antigen particles exposing highly conserved E2 epitope of hepatitis C virus.
Czarnota A, Tyborowska J, Peszyńska-Sularz G, Gromadzka B, Bieńkowska-Szewczyk K, Grzyb K. Czarnota A, et al. Microb Cell Fact. 2016 Apr 13;15:62. doi: 10.1186/s12934-016-0460-4. Microb Cell Fact. 2016. PMID: 27075377 Free PMC article. - Expression and purification of hepatitis B surface antigen S from Escherichia coli; a new simple method.
Elghanam MS, Attia AS, Shoeb HA, Hashem AE. Elghanam MS, et al. BMC Res Notes. 2012 Mar 1;5:125. doi: 10.1186/1756-0500-5-125. BMC Res Notes. 2012. PMID: 22381042 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources