Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase - PubMed (original) (raw)
. 1980 Nov 6;288(5786):98-100.
doi: 10.1038/288098a0.
- PMID: 6159548
- DOI: 10.1038/288098a0
Interferon-dependent induction of mRNA activity for (2'-5')oligo-isoadenylate synthetase
L Shulman et al. Nature. 1980.
Abstract
At least three different enzymes involved in the regulation of protein synthesis are induced in a variety of cells by interferon (IFN). Sensitive assays for these enzymes have been developed and used to establish the specificity, dose dependence and time course of their induction by IFN. One of these enzymes, the oligo-isoadenylate synthetase E, whose product (2'-5')pppApApA activates the latent ribonuclease F, is increased over 50-fold after IFN treatment. We describe here the assay for an mRNA from IFN-treated mouse L cells, that produces oligo-isoadenylate synthetase activity when injected into Xenopus oocytes. This mRNA is found in the cells only after exposure to IFN. The mRNA increases in mouse L cells with the same time course as the enzyme activity itself. In particular, there is a 3-h lag period between IFN addition and the onset of enzyme and mRNA accumulation. Using anti-IFN antibodies, we show that during this lag period the continued interaction of IFN with the cells is necessary for the full induction of the oligo-isoadenylate synthetase.
Similar articles
- [Identification of the isoadenylate synthetase in the messenger RNA translation products of antiviral proteins].
Sokolova TM, Kisling W, Khil'ko SN, Fel'gengaueér PE, Tazulakhova EB. Sokolova TM, et al. Vopr Virusol. 1981 May-Jun;(3):339-47. Vopr Virusol. 1981. PMID: 6170176 Russian. - Cell-regulatory functions of interferon induced enzymes: antimitogenic effect of (2'-5')oligo-A, growth-related variations in (2'-5')oligo-A synthetase, and isolation of its mRNA.
Revel M, Kimchi A, Friedman M, Wolf D, Merlin G, Panet A, Rapoport S, Lapidot Y. Revel M, et al. Tex Rep Biol Med. 1981-1982;41:452-62. Tex Rep Biol Med. 1981. PMID: 6189209 Review. No abstract available. - Enzymology of interferon action. The (2'-5')(A)n synthetase-RNase L pathway.
Dougherty JP, Samanta H, Floyd-Smith G, Broeze R, Jayaram BM, Lengyel P. Dougherty JP, et al. Tex Rep Biol Med. 1981-1982;41:443-51. Tex Rep Biol Med. 1981. PMID: 6189208 Review. No abstract available.
Cited by
- The beta2-microglobulin mRNA in human Daudi cells has a mutated initiation codon but is still inducible by interferon.
Rosa F, Berissi H, Weissenbach J, Maroteaux L, Fellous M, Revel M. Rosa F, et al. EMBO J. 1983;2(2):239-43. doi: 10.1002/j.1460-2075.1983.tb01412.x. EMBO J. 1983. PMID: 11894933 Free PMC article. - Modulation of tubulin mRNA levels by interferon in human lymphoblastoid cells.
Fellous A, Ginzburg I, Littauer UZ. Fellous A, et al. EMBO J. 1982;1(7):835-9. doi: 10.1002/j.1460-2075.1982.tb01256.x. EMBO J. 1982. PMID: 6964957 Free PMC article. - Biosynthesis and secretion of catalytically active acetylcholinesterase in Xenopus oocytes microinjected with mRNA from rat brain and from Torpedo electric organ.
Soreq H, Parvari R, Silman I. Soreq H, et al. Proc Natl Acad Sci U S A. 1982 Feb;79(3):830-4. doi: 10.1073/pnas.79.3.830. Proc Natl Acad Sci U S A. 1982. PMID: 6950429 Free PMC article. - Molecular cloning and sequence of partial cDNA for interferon-induced (2'-5')oligo(A) synthetase mRNA from human cells.
Merlin G, Chebath J, Benech P, Metz R, Revel M. Merlin G, et al. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4904-8. doi: 10.1073/pnas.80.16.4904. Proc Natl Acad Sci U S A. 1983. PMID: 6348777 Free PMC article. - Cyclic AMP-dependent regulation of activities of synthetase and phosphodiesterase of 2',5'-oligoadenylate in NIH 3T3 cells.
Itkes AV, Turpaev KT, Kartasheva ON, Kafiani CA, Severin ES. Itkes AV, et al. Mol Cell Biochem. 1984;58(1-2):165-71. doi: 10.1007/BF00240616. Mol Cell Biochem. 1984. PMID: 6323957
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources