Progressive loss of shape-responsive metabolic controls in cells with increasingly transformed phenotype - PubMed (original) (raw)
Progressive loss of shape-responsive metabolic controls in cells with increasingly transformed phenotype
S C Wittelsberger et al. Cell. 1981 Jun.
Abstract
The modulation of cell metabolism by cell shape and external surface contact has been studied by suspension culture of anchorage-dependent fibroblasts. The suspended cells shut down protein synthesis and nuclear RNA metabolism and cease replicating DNA. However, these responses to suspension are lost or modified as cells become progressively transformed in behavior. We compare the metabolic consequences of suspension culture in five related types of fibroblasts: the well regulated mouse diploid fibroblast; the spontaneously immortalized and progressively less well regulated lines 3T3, 3T6 and HDP3T6; and the fully transformed anchorage-independent SVPy3T3. Protein synthesis is inhibited rapidly following suspension in diploid fibroblasts and more slowly in the less well regulated cells. In contrast, the response of hnRNA synthesis to suspension is lost completely when cells adopt the 3T6 phenotype, and message regulation is lost in HDP3T6. The prompt inhibition of ribosomal RNA precursor is modified to a slow decline in HDP3T6. The metabolism of fully transformed SVPy3T3 cells is indifferent to suspension. The progressive loss of shape-responsive controls may be related to tumor progression.
Similar articles
- Cell cycle perturbations in normal and transformed fibroblasts caused by detachment from the substratum.
Campisi J, Medrano EE. Campisi J, et al. J Cell Physiol. 1983 Jan;114(1):53-60. doi: 10.1002/jcp.1041140109. J Cell Physiol. 1983. PMID: 6186678 No abstract available. - Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts.
Ben-Ze'ev A, Farmer SR, Penman S. Ben-Ze'ev A, et al. Cell. 1980 Sep;21(2):365-72. doi: 10.1016/0092-8674(80)90473-0. Cell. 1980. PMID: 6157481 - The relative amounts of the cytoplasmic RNA species in normal, transformed and senescent cultured cell lines.
Johnson LF, Abelson HT, Penman S, Green H. Johnson LF, et al. J Cell Physiol. 1977 Mar;90(3):465-70. doi: 10.1002/jcp.1040900310. J Cell Physiol. 1977. PMID: 192738 - Multinucleation and inhibition of cytokinesis in suspended cells: reversal upon reattachment to a substrate.
Ben-Ze'ev A, Raz A. Ben-Ze'ev A, et al. Cell. 1981 Oct;26(1 Pt 1):107-15. doi: 10.1016/0092-8674(81)90038-6. Cell. 1981. PMID: 6799205 - [HPV16 participates in progressive transformation of normal epidermal cells].
Yasumoto S. Yasumoto S. Gan To Kagaku Ryoho. 1989 Mar;16(3 Pt 2):549-61. Gan To Kagaku Ryoho. 1989. PMID: 2539784 Review. Japanese.
Cited by
- Transient induction of vinculin gene expression in 3T3 fibroblasts stimulated by serum-growth factors.
Ben-Ze'ev A, Reiss R, Bendori R, Gorodecki B. Ben-Ze'ev A, et al. Cell Regul. 1990 Aug;1(9):621-36. doi: 10.1091/mbc.1.9.621. Cell Regul. 1990. PMID: 2127699 Free PMC article. - Cell configuration-related control of vimentin biosynthesis and phosphorylation in cultured mammalian cells.
Ben-Ze'ev A. Ben-Ze'ev A. J Cell Biol. 1983 Sep;97(3):858-65. doi: 10.1083/jcb.97.3.858. J Cell Biol. 1983. PMID: 6885922 Free PMC article. - Cell-contact and -architecture of malignant cells and their relationship to metastasis.
Raz A, Ben-Ze'ev A. Raz A, et al. Cancer Metastasis Rev. 1987;6(1):3-21. doi: 10.1007/BF00047606. Cancer Metastasis Rev. 1987. PMID: 3297370 Review. - Anchorage-dependent control of muscle-specific gene expression in C2C12 mouse myoblasts.
Milasincic DJ, Dhawan J, Farmer SR. Milasincic DJ, et al. In Vitro Cell Dev Biol Anim. 1996 Feb;32(2):90-9. doi: 10.1007/BF02723040. In Vitro Cell Dev Biol Anim. 1996. PMID: 8907122 - G1/S control of anchorage-independent growth in the fibroblast cell cycle.
Guadagno TM, Assoian RK. Guadagno TM, et al. J Cell Biol. 1991 Dec;115(5):1419-25. doi: 10.1083/jcb.115.5.1419. J Cell Biol. 1991. PMID: 1955482 Free PMC article.