Location and distribution of the light chains in clathrin trimers - PubMed (original) (raw)

Location and distribution of the light chains in clathrin trimers

T Kirchhausen et al. Proc Natl Acad Sci U S A. 1983 May.

Abstract

Mouse monoclonal antibodies have been made that are specific for one of the light chains (LC-A) of calf brain clathrin. The determinant they recognize has been mapped by immunoelectron microscopy and shown to lie near the center of a clathrin trimer. Quantitative immunoprecipitation experiments with one of the monoclonal antibodies suggests that, despite the overall 1:2 stoichiometry, the light chain composition of an individual clathrin trimer is nearly random.

PubMed Disclaimer

Similar articles

• Mapping two functional domains of clathrin light chains with monoclonal antibodies.
  Kohtz DS, Georgieva-Hanson V, Kohtz JD, Schook WJ, Puszkin S. Kohtz DS, et al. J Cell Biol. 1987 Apr;104(4):897-903. doi: 10.1083/jcb.104.4.897. J Cell Biol. 1987. PMID: 2435741 Free PMC article.
• Polymorphism in clathrin light chains from different tissues.
  Brodsky FM, Parham P. Brodsky FM, et al. J Mol Biol. 1983 Jun 15;167(1):197-204. doi: 10.1016/s0022-2836(83)80041-2. J Mol Biol. 1983. PMID: 6864801
• Protein organization in clathrin trimers.
  Kirchhausen T, Harrison SC. Kirchhausen T, et al. Cell. 1981 Mar;23(3):755-61. doi: 10.1016/0092-8674(81)90439-6. Cell. 1981. PMID: 7226229
• Structural domains of clathrin heavy chains.
  Kirchhausen T, Harrison SC. Kirchhausen T, et al. J Cell Biol. 1984 Nov;99(5):1725-34. doi: 10.1083/jcb.99.5.1725. J Cell Biol. 1984. PMID: 6386825 Free PMC article.

Cited by

• Imaging vesicle formation dynamics supports the flexible model of clathrin-mediated endocytosis.
  Nawara TJ, Williams YD 2nd, Rao TC, Hu Y, Sztul E, Salaita K, Mattheyses AL. Nawara TJ, et al. Nat Commun. 2022 Apr 1;13(1):1732. doi: 10.1038/s41467-022-29317-1. Nat Commun. 2022. PMID: 35365614 Free PMC article.
• Clathrin Light Chains: Not to Be Taken so Lightly.
  Das J, Tiwari M, Subramanyam D. Das J, et al. Front Cell Dev Biol. 2021 Dec 14;9:774587. doi: 10.3389/fcell.2021.774587. eCollection 2021. Front Cell Dev Biol. 2021. PMID: 34970544 Free PMC article. Review.
• Clathrin light chain diversity regulates membrane deformation in vitro and synaptic vesicle formation in vivo.
  Redlingshöfer L, McLeod F, Chen Y, Camus MD, Burden JJ, Palomer E, Briant K, Dannhauser PN, Salinas PC, Brodsky FM. Redlingshöfer L, et al. Proc Natl Acad Sci U S A. 2020 Sep 22;117(38):23527-23538. doi: 10.1073/pnas.2003662117. Epub 2020 Sep 9. Proc Natl Acad Sci U S A. 2020. PMID: 32907943 Free PMC article.
• Taming the Triskelion: Bacterial Manipulation of Clathrin.
  Latomanski EA, Newton HJ. Latomanski EA, et al. Microbiol Mol Biol Rev. 2019 Feb 27;83(2):e00058-18. doi: 10.1128/MMBR.00058-18. Print 2019 May 15. Microbiol Mol Biol Rev. 2019. PMID: 30814130 Free PMC article. Review.
• Crosstalk between CLCb/Dyn1-Mediated Adaptive Clathrin-Mediated Endocytosis and Epidermal Growth Factor Receptor Signaling Increases Metastasis.
  Chen PH, Bendris N, Hsiao YJ, Reis CR, Mettlen M, Chen HY, Yu SL, Schmid SL. Chen PH, et al. Dev Cell. 2017 Feb 6;40(3):278-288.e5. doi: 10.1016/j.devcel.2017.01.007. Dev Cell. 2017. PMID: 28171750 Free PMC article.

References

1. 1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
2. 1. J Mol Biol. 1975 Sep 5;97(1):93-8 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1979 Jan;76(1):116-20 - PubMed
4. 1. Cell. 1979 Feb;16(2):303-12 - PubMed
5. 1. Nature. 1978 Nov 16;276(5685):269-70 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations

• Research Materials

  • Cellosaurus - a cell line knowledge resource
  • NCI CPTC Antibody Characterization Program