Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3 - PubMed (original) (raw)
. 1983 Aug;304(5925):459-62.
doi: 10.1038/304459a0.
- PMID: 6192344
- DOI: 10.1038/304459a0
Critical role of an eight-amino acid sequence of VP1 in neutralization of poliovirus type 3
D M Evans et al. Nature. 1983 Aug.
Abstract
The three serotypes of poliovirus are members of the picornaviradae, a group of viruses which cause a variety of diseases of considerable importance in man and animals. We have previously used antigenic mutants resistant to neutralizing monoclonal antibodies to identify a single antigenic site for the neutralization of poliovirus type 3 (ref. 1). Evidence based on oligonucleotide mapping suggested that this site corresponded largely to one physicochemical region of the capsid protein viral polypeptide 1 (VP1). We now present conclusive evidence that most of the mutations conferring resistance to neutralization are confined to an eight-amino acid region of VP1, specified by a sequence of viral RNA 277-300 bases from the start of the region coding for VP1. These data strongly suggest that this small region constitutes a major antigenic site involved in virus neutralization and they provide the most detailed information currently available on the antigenic site of a human virus.
Similar articles
- Principal and subsidiary antigenic sites of VP1 involved in the neutralization of poliovirus type 3.
Minor PD, Evans DM, Ferguson M, Schild GC, Westrop G, Almond JW. Minor PD, et al. J Gen Virol. 1985 May;66 ( Pt 5):1159-65. doi: 10.1099/0022-1317-66-5-1159. J Gen Virol. 1985. PMID: 2582084 - Location and primary structure of a major antigenic site for poliovirus neutralization.
Minor PD, Schild GC, Bootman J, Evans DM, Ferguson M, Reeve P, Spitz M, Stanway G, Cann AJ, Hauptmann R, Clarke LD, Mountford RC, Almond JW. Minor PD, et al. Nature. 1983 Feb 24;301(5902):674-9. doi: 10.1038/301674a0. Nature. 1983. PMID: 6186919 - Application of monoclonal antibodies for the identification of the antigenic site for poliovirus neutralization.
Minor PD, Schild GC, Evans D, Ferguson M, Almond J. Minor PD, et al. Dev Biol Stand. 1984;57:171-5. Dev Biol Stand. 1984. PMID: 6084608 - [Structural polypeptides and antigenicity of poliovirus].
Taniguchi K, Urasawa S. Taniguchi K, et al. Uirusu. 1985 Jun;35(1):17-34. doi: 10.2222/jsv.35.17. Uirusu. 1985. PMID: 2414923 Review. Japanese. No abstract available. - Structure of viral B-cell epitopes.
Van Regenmortel MH. Van Regenmortel MH. Res Microbiol. 1990 Sep-Oct;141(7-8):747-56. doi: 10.1016/0923-2508(90)90106-z. Res Microbiol. 1990. PMID: 1714092 Review.
Cited by
- Regions of poliovirus protein VP1 produced in Escherichia coli induce neutralizing antibodies.
Hoatlin ME, Kew OM, Renz ME. Hoatlin ME, et al. J Virol. 1987 May;61(5):1442-7. doi: 10.1128/JVI.61.5.1442-1447.1987. J Virol. 1987. PMID: 3033273 Free PMC article. - Very high frequency of reversion to guanidine resistance in clonal pools of guanidine-dependent type 1 poliovirus.
de la Torre JC, Wimmer E, Holland JJ. de la Torre JC, et al. J Virol. 1990 Feb;64(2):664-71. doi: 10.1128/JVI.64.2.664-671.1990. J Virol. 1990. PMID: 2153232 Free PMC article. - Restricted virus replication in the spinal cords of nude mice infected with a Theiler's virus variant.
Zurbriggen A, Yamada M, Thomas C, Fujinami RS. Zurbriggen A, et al. J Virol. 1991 Feb;65(2):1023-30. doi: 10.1128/JVI.65.2.1023-1030.1991. J Virol. 1991. PMID: 1987366 Free PMC article. - Defining the Enterovirus Diversity Landscape of a Fecal Sample: A Methodological Challenge?
Faleye TO, Adewumi MO, Adeniji JA. Faleye TO, et al. Viruses. 2016 Jan 12;8(1):18. doi: 10.3390/v8010018. Viruses. 2016. PMID: 26771630 Free PMC article. Review. - Evidence for at least two dominant neutralization antigens on human rhinovirus 14.
Sherry B, Rueckert R. Sherry B, et al. J Virol. 1985 Jan;53(1):137-43. doi: 10.1128/JVI.53.1.137-143.1985. J Virol. 1985. PMID: 2981332 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources