India ink staining of proteins on nitrocellulose paper - PubMed (original) (raw)
India ink staining of proteins on nitrocellulose paper
K Hancock et al. Anal Biochem. 1983 Aug.
Abstract
India ink staining of proteins that have been electrotransfer blotted onto nitrocellulose paper is described. This stain proved to be a useful adjunct to the enzyme-linked immunoelectrotransfer blot technique. It is more sensitive than Coomassie blue, amido black, and fast green stains and is simple to use.
Similar articles
- Comparison of the sensitivity of different India inks staining of electro-blotted proteins on filter membranes.
Lek LH, Yang EB, Wang DF, Cheng LY. Lek LH, et al. J Biochem Biophys Methods. 1995 Feb;30(1):9-20. doi: 10.1016/0165-022x(94)00059-m. J Biochem Biophys Methods. 1995. PMID: 7541816 - Fixation increases sensitivity of India ink staining of proteins and peptides on nitrocellulose paper.
Li KW, Geraerts WP, van Elk R, Joosse J. Li KW, et al. Anal Biochem. 1988 Oct;174(1):97-100. doi: 10.1016/0003-2697(88)90523-4. Anal Biochem. 1988. PMID: 2464292 - Detection of proteins on blot transfer membranes.
Sasse J, Gallagher SR. Sasse J, et al. Curr Protoc Immunol. 2008 Nov;Chapter 8:8.10B.1-8.10B.6. doi: 10.1002/0471142735.im0810bs83. Curr Protoc Immunol. 2008. PMID: 19016450 - Solubilization of protein-dye complexes on nitrocellulose to quantify proteins spectrophotometrically.
Goldring JP, Ravaioli L. Goldring JP, et al. Anal Biochem. 1996 Nov 15;242(2):197-201. doi: 10.1006/abio.1996.0453. Anal Biochem. 1996. PMID: 8937562 - Staining proteins in gels.
Sasse J, Gallagher SR. Sasse J, et al. Curr Protoc Immunol. 2004 Feb;Chapter 8:Unit 8.9. doi: 10.1002/0471142735.im0809s58. Curr Protoc Immunol. 2004. PMID: 18432935 Review.
Cited by
- Isolation and purification of a cell adhesion factor from crayfish blood cells.
Johansson MW, Söderhäll K. Johansson MW, et al. J Cell Biol. 1988 May;106(5):1795-803. doi: 10.1083/jcb.106.5.1795. J Cell Biol. 1988. PMID: 2453523 Free PMC article. - Immunological diagnosis of human hydatid cyst relapse: utility of the enzyme-linked immunoelectrotransfer blot and discriminant analysis.
Gadea I, Ayala G, Diago MT, Cuñat A, Garcia de Lomas J. Gadea I, et al. Clin Diagn Lab Immunol. 2000 Jul;7(4):549-52. doi: 10.1128/CDLI.7.4.549-552.2000. Clin Diagn Lab Immunol. 2000. PMID: 10882649 Free PMC article. - Detection of CaMV gene I and gene VI protein products in vivo using antisera raised to COOH-terminal β-galactosidase fusion proteins.
Harker CL, Mullineaux PM, Bryant JA, Maule AJ. Harker CL, et al. Plant Mol Biol. 1987 May;8(3):275-87. doi: 10.1007/BF00015035. Plant Mol Biol. 1987. PMID: 24301131 - Phosphorylation of the hepatitis delta virus antigens.
Bichko V, Barik S, Taylor J. Bichko V, et al. J Virol. 1997 Jan;71(1):512-8. doi: 10.1128/JVI.71.1.512-518.1997. J Virol. 1997. PMID: 8985379 Free PMC article. - Domain organization of the yeast histone chaperone FACT: the conserved N-terminal domain of FACT subunit Spt16 mediates recovery from replication stress.
O'Donnell AF, Brewster NK, Kurniawan J, Minard LV, Johnston GC, Singer RA. O'Donnell AF, et al. Nucleic Acids Res. 2004 Nov 1;32(19):5894-906. doi: 10.1093/nar/gkh922. Print 2004. Nucleic Acids Res. 2004. PMID: 15520471 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources