Characterisation of a mutant from Escherichia coli lacking protein L15 and localisation of protein L15 by immuno-electron microscopy - PubMed (original) (raw)

• PMID: 6197616
• DOI: 10.1007/BF00392165

Characterisation of a mutant from Escherichia coli lacking protein L15 and localisation of protein L15 by immuno-electron microscopy

M Lotti et al. Mol Gen Genet. 1983.

Abstract

Two mutants lacking protein L15 from the ribosome as determined by two dimensional gels were investigated using a number of different immunological methods. One strain was found to possess several protein L15 moieties which differed in net charge and in size. The other showed no evidence of L15 cross-reacting material (CRM) on the ribosome or in the supernatant. Ribosomes of this strain were used as a control in the process of the localisation of protein L15 on the surface of the large subunit of Escherichia coli ribosomes. Antigenic determinants mapped in the angle between the central protuberance and the L1 protuberance. Protein L15 has been assigned a central role in the large subunit in vitro assembly map, in peptidyltransferase activity and in the binding of erythromycin, so the significance of a mutant lacking this protein is discussed.

PubMed Disclaimer

Similar articles

• Immunological studies of Escherichia coli mutants lacking one or two ribosomal proteins.
  Dabbs ER, Hasenbank R, Kastner B, Rak KH, Wartusch B, Stöffler G. Dabbs ER, et al. Mol Gen Genet. 1983;192(3):301-8. doi: 10.1007/BF00392166. Mol Gen Genet. 1983. PMID: 6361486
• Mutants of Escherichia coli lacking ribosomal protein L1.
  Dabbs ER, Ehrlich R, Hasenbank R, Schroeter BH, Stöffler-Meilicke M, Stöffler G. Dabbs ER, et al. J Mol Biol. 1981 Jul 15;149(4):553-78. doi: 10.1016/0022-2836(81)90347-8. J Mol Biol. 1981. PMID: 6171646 No abstract available.
• A mutant from Escherichia coli which lacks ribosomal proteins S17 and L29 used to localize these two proteins on the ribosomal surface.
  Stöffler-Meilicke M, Dabbs ER, Albrecht-Ehrlich R, Stöffler G. Stöffler-Meilicke M, et al. Eur J Biochem. 1985 Aug 1;150(3):485-90. doi: 10.1111/j.1432-1033.1985.tb09048.x. Eur J Biochem. 1985. PMID: 3926498
• The localization of protein L19 on the surface of 50 S subunits of Escherichia coli aided by the use of mutants lacking protein L19.
  Stöffler G, Noah M, Stöffler-Meilicke M, Dabbs ER. Stöffler G, et al. J Biol Chem. 1984 Apr 10;259(7):4521-6. J Biol Chem. 1984. PMID: 6423642
• Bacterial surface proteins. Some structural, functional and evolutionary aspects.
  Baumeister W, Wildhaber I, Engelhardt H. Baumeister W, et al. Biophys Chem. 1988 Feb;29(1-2):39-49. doi: 10.1016/0301-4622(88)87023-6. Biophys Chem. 1988. PMID: 3129041 Review.

Cited by

• A pair of Bacillus subtilis ribosomal protein genes mapping outside the principal ribosomal protein cluster.
  Dabbs ER. Dabbs ER. J Bacteriol. 1983 Nov;156(2):966-9. doi: 10.1128/jb.156.2.966-969.1983. J Bacteriol. 1983. PMID: 6415045 Free PMC article.
• Structure and regulation of a nuclear gene in Saccharomyces cerevisiae that specifies MRP13, a protein of the small subunit of the mitochondrial ribosome.
  Partaledis JA, Mason TL. Partaledis JA, et al. Mol Cell Biol. 1988 Sep;8(9):3647-60. doi: 10.1128/mcb.8.9.3647-3660.1988. Mol Cell Biol. 1988. PMID: 3065621 Free PMC article.
• Ribosomal protein L30 is dispensable in the yeast Saccharomyces cerevisiae.
  Baronas-Lowell DM, Warner JR. Baronas-Lowell DM, et al. Mol Cell Biol. 1990 Oct;10(10):5235-43. doi: 10.1128/mcb.10.10.5235-5243.1990. Mol Cell Biol. 1990. PMID: 2204809 Free PMC article.
• A model for the spatial arrangement of the proteins in the large subunit of the Escherichia coli ribosome.
  Walleczek J, Schüler D, Stöffler-Meilicke M, Brimacombe R, Stöffler G. Walleczek J, et al. EMBO J. 1988 Nov;7(11):3571-6. doi: 10.1002/j.1460-2075.1988.tb03234.x. EMBO J. 1988. PMID: 2463164 Free PMC article.
• The binding site for ribosomal protein L2 within 23S ribosomal RNA of Escherichia coli.
  Beauclerk AA, Cundliffe E. Beauclerk AA, et al. EMBO J. 1988 Nov;7(11):3589-94. doi: 10.1002/j.1460-2075.1988.tb03236.x. EMBO J. 1988. PMID: 3061801 Free PMC article.

References

1. 1. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6652-6 - PubMed
2. 1. J Biol Chem. 1977 Oct 25;252(20):7323-36 - PubMed
3. 1. Proc Natl Acad Sci U S A. 1983 Nov;80(22):6780-4 - PubMed
4. 1. J Biol Chem. 1982 Mar 10;257(5):2649-56 - PubMed
5. 1. Proc Natl Acad Sci U S A. 1971 Sep;68(9):2283-7 - PubMed

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Springer