Participation of membrane-associated proteins in the formation of the cross-linked envelope of the keratinocyte - PubMed (original) (raw)
Participation of membrane-associated proteins in the formation of the cross-linked envelope of the keratinocyte
M Simon et al. Cell. 1984 Apr.
Abstract
Cultured keratinocytes, like those in natural squamous epithelia, form submembranous protein envelopes cross-linked by cellular transglutaminase. During the cross-linking, the cytosolic protein involucrin becomes incorporated into the envelope and can no longer be extracted by detergents. We show here that when transglutaminase is activated in cultured keratinocytes, at least six other proteins also become nonextractable. In contrast to involucrin, these proteins are associated with membranes. Two of the proteins (210 and 195 kd) are differentiated products specific to the keratinocyte; like involucrin, they are absent from small keratinocytes and fibroblasts, but appear in larger keratinocytes during the course of their terminal differentiation. The other proteins that become nonextractable cannot be destined exclusively for envelope formation since they are also present in fibroblasts. Transglutaminase is used by the mature (large) keratinocyte to make a detergent-resistant envelope from what appears to be a mixture of differentiation-specific and nonspecific proteins, both membrane-bound and cytosolic.
Similar articles
- Enzymatic cross-linking of involucrin and other proteins by keratinocyte particulates in vitro.
Simon M, Green H. Simon M, et al. Cell. 1985 Mar;40(3):677-83. doi: 10.1016/0092-8674(85)90216-8. Cell. 1985. PMID: 2578890 - Cross-linked envelope-related markers for squamous differentiation in human lung cancer cell lines.
Levitt ML, Gazdar AF, Oie HK, Schuller H, Thacher SM. Levitt ML, et al. Cancer Res. 1990 Jan 1;50(1):120-8. Cancer Res. 1990. PMID: 1967140 - Biochemistry of transglutaminases and cross-linking in the skin.
Peterson LL, Zettergren JG, Wuepper KD. Peterson LL, et al. J Invest Dermatol. 1983 Jul;81(1 Suppl):95s-100s. doi: 10.1111/1523-1747.ep12540777. J Invest Dermatol. 1983. PMID: 6134777 Review. - Structural organization of cornified cell envelopes and alterations in inherited skin disorders.
Ishida-Yamamoto A, Iizuka H. Ishida-Yamamoto A, et al. Exp Dermatol. 1998 Feb;7(1):1-10. doi: 10.1111/j.1600-0625.1998.tb00295.x. Exp Dermatol. 1998. PMID: 9517915 Review.
Cited by
- Early Transcriptional Changes of Adipose-Derived Stem Cells (ADSCs) in Cell Culture.
Taha S, Akova E, Saller MM, Giunta RE, Haas-Lützenberger EM. Taha S, et al. Medicina (Kaunas). 2022 Sep 9;58(9):1249. doi: 10.3390/medicina58091249. Medicina (Kaunas). 2022. PMID: 36143926 Free PMC article. - Prognostic implications of PPL expression in ovarian cancer.
Hua T, Zhao BB, Fan SB, Zhao CF, Kong YH, Tian RQ, Zhang BY. Hua T, et al. Discov Oncol. 2022 May 25;13(1):35. doi: 10.1007/s12672-022-00496-z. Discov Oncol. 2022. PMID: 35612641 Free PMC article. - Loricrin and NRF2 Coordinate Cornification.
Ishitsuka Y, Ogawa T, Nakamura Y, Kubota N, Fujisawa Y, Watanabe R, Okiyama N, Fujimoto M, Roop DR, Ishida-Yamamoto A. Ishitsuka Y, et al. JID Innov. 2021 Oct 15;2(1):100065. doi: 10.1016/j.xjidi.2021.100065. eCollection 2022 Jan. JID Innov. 2021. PMID: 35024686 Free PMC article. - Mammalian Plakins, Giant Cytolinkers: Versatile Biological Functions and Roles in Cancer.
Hu L, Huang Z, Wu Z, Ali A, Qian A. Hu L, et al. Int J Mol Sci. 2018 Mar 24;19(4):974. doi: 10.3390/ijms19040974. Int J Mol Sci. 2018. PMID: 29587367 Free PMC article. Review. - The Molecular Revolution in Cutaneous Biology: EDC and Locus Control.
Oh IY, de Guzman Strong C. Oh IY, et al. J Invest Dermatol. 2017 May;137(5):e101-e104. doi: 10.1016/j.jid.2016.03.046. J Invest Dermatol. 2017. PMID: 28411839 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources