Anti-hypervariable region antibody induced by a defined peptide: an approach for studying the structural correlates of idiotypes - PubMed (original) (raw)

Anti-hypervariable region antibody induced by a defined peptide: an approach for studying the structural correlates of idiotypes

P P Chen et al. Proc Natl Acad Sci U S A. 1984 Mar.

Abstract

The structural correlates of idiotypes have been sought in several antibody systems. However, the precise molecular basis of idiotypes are exceedingly difficult to define. Antibodies of predetermined specificity can be induced by immunization with synthetic peptides. In the present experiments, a synthetic peptide corresponding to a hypervariable region on a monoclonal human IgM rheumatoid factor (Sie) has been used to induce specific anti-hypervariable region antibodies. The antibodies bound to the intact Ig molecule and to its isolated heavy chains but not to other IgM paraproteins nor to pooled human IgG. The binding of the antibody to the intact IgM was inhibited specifically by the free peptide, and the antibody activity was removed by a peptide-coupled affinity column. These results clearly demonstrate that specific anti-idiotypic antibody of predefined specificity can be induced by a hypervariable region peptide. Antibodies of this class may provide a new tool for defining the structural correlates of idiotypes.

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Genetic basis for the cross-reactive idiotypes on the light chains of human IgM anti-IgG autoantibodies.
Chen PP, Albrandt K, Orida NK, Radoux V, Chen EY, Schrantz R, Liu FT, Carson DA. Chen PP, et al. Proc Natl Acad Sci U S A. 1986 Nov;83(21):8318-22. doi: 10.1073/pnas.83.21.8318. Proc Natl Acad Sci U S A. 1986. PMID: 3095834 Free PMC article.
Construction of an extended three-dimensional idiotope map by electron microscopic analysis of idiotope-anti-idiotope complexes.
Roux KH, Monafo WJ, Davie JM, Greenspan NS. Roux KH, et al. Proc Natl Acad Sci U S A. 1987 Jul;84(14):4984-8. doi: 10.1073/pnas.84.14.4984. Proc Natl Acad Sci U S A. 1987. PMID: 3496600 Free PMC article.
Cross-reacting idiotypes on cryoprecipitating rheumatoid factor.
Chen PP, Fong S, Goni F, Silverman GJ, Fox RI, Liu MF, Frangione B, Carson DA. Chen PP, et al. Springer Semin Immunopathol. 1988;10(1):35-55. doi: 10.1007/BF02054022. Springer Semin Immunopathol. 1988. PMID: 3137675 Review. No abstract available.
Cloning and sequence determination of a human rheumatoid factor light-chain gene.
Jirik FR, Sorge J, Fong S, Heitzmann JG, Curd JG, Chen PP, Goldfien R, Carson DA. Jirik FR, et al. Proc Natl Acad Sci U S A. 1986 Apr;83(7):2195-9. doi: 10.1073/pnas.83.7.2195. Proc Natl Acad Sci U S A. 1986. PMID: 3083417 Free PMC article.
Characterization of human rheumatoid factors with seven antiidiotypes induced by synthetic hypervariable region peptides.
Chen PP, Gõni F, Houghten RA, Fong S, Goldfien R, Vaughan JH, Frangione B, Carson DA. Chen PP, et al. J Exp Med. 1985 Aug 1;162(2):487-500. doi: 10.1084/jem.162.2.487. J Exp Med. 1985. PMID: 2410527 Free PMC article.

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Anti-hypervariable region antibody induced by a defined peptide: an approach for studying the structural correlates of idiotypes - PubMed (original) (raw)