Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors - PubMed (original) (raw)
Lariat RNA's as intermediates and products in the splicing of messenger RNA precursors
R A Padgett et al. Science. 1984.
Abstract
The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.
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