Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus - PubMed (original) (raw)

Compartmentation of asparagine-linked oligosaccharide processing in the Golgi apparatus

W G Dunphy et al. J Cell Biol. 1983 Jul.

Abstract

Golgi-associated processing of complex-type oligosaccharides linked to asparagine involves the sequential action of at least six enzymes. By equilibrium sucrose density gradient centrifugation of membranes from Chinese hamster ovary cells, we have partially resolved the set of four initial enzymes in the pathway (Mannosidase I, N-acetylglucosamine (GlcNAc) Transferase I, Mannosidase II, and GlcNAc Transferase II) from two later-acting activities (galactosyltransferase and sialyltransferase). In view of the recent demonstration that galactosyltransferase is restricted to the trans face of the Golgi complex in HeLa cells (Roth, J., and E.G. Berger, 1982, J. Cell Biol., 93:223-229), our results suggest that removal of mannose and attachment of peripheral N-acetylglucosamine may occur in some or all of the remaining cisternae on the cis side of the Golgi stack.

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References

1. 1. J Biol Chem. 1982 May 25;257(10):5323-5 - PubMed
2. 1. J Biol Chem. 1977 Jun 10;252(11):3926-33 - PubMed
3. 1. J Cell Biol. 1973 Oct;59(1):73-88 - PubMed
4. 1. J Biol Chem. 1980 Mar 25;255(6):2255-8 - PubMed
5. 1. J Biol Chem. 1977 Feb 10;252(3):1107-16 - PubMed

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