Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells - PubMed (original) (raw)

Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells

H Trachsel et al. Proc Natl Acad Sci U S A. 1980 Feb.

Abstract

It was previously shown that the poliovirus-induced inhibition of translation of capped mRNAs can be reversed by a protein found in preparations of the eukaryotic initiation factor eIF-4B [Rose, J. K., Trachsel, H., Leong, K. & Baltimore, D. (1978) Proc. Natl. Acad. Sci. USA 75, 2732--2736]. This "restoring factor" has now been purified from a high-salt wash of rabbit reticulocyte ribosomes by taking advantage of its tight association with factor eIF-3 at low salt concentrations. It did not copurify with the major Mr 80,000 polypeptide of eIF-4B preparations but did copurify with a Mr 24,000 polypeptide previously shown to bind to the cap structures of mRNAs [Sonenberg, N., Rupprecht, K. M., Hecht, S. M. & Shatkin, A. J. (1979) Proc. Natl. Acad. Sci. USA 76, 4345--4349]. Both the electrophoretic mobility and the tryptic peptide pattern of the restoring factor were indistinguishable from those of the cap-binding protein, and the restoring factor could be crosslinked to the 5'-terminal cap on mRNA. Thus, is appears that poliovirus inhibits cellular protein synthesis by inactivation of some crucial property of the cap-binding protein.

PubMed Disclaimer

Cited by

eIF4F: a retrospective.
Merrick WC. Merrick WC. J Biol Chem. 2015 Oct 2;290(40):24091-9. doi: 10.1074/jbc.R115.675280. Epub 2015 Aug 31. J Biol Chem. 2015. PMID: 26324716 Free PMC article. Review.
Selective human enterovirus and rhinovirus inhibitors: An overview of capsid-binding and protease-inhibiting molecules.
Shih SR, Chen SJ, Hakimelahi GH, Liu HJ, Tseng CT, Shia KS. Shih SR, et al. Med Res Rev. 2004 Jul;24(4):449-74. doi: 10.1002/med.10067. Med Res Rev. 2004. PMID: 15170592 Free PMC article. Review.
Localization of cap-binding protein in subcellular fractions of HeLa cells.
Hansen JL, Etchison DO, Hershey JW, Ehrenfeld E. Hansen JL, et al. Mol Cell Biol. 1982 Dec;2(12):1639-43. doi: 10.1128/mcb.2.12.1639-1643.1982. Mol Cell Biol. 1982. PMID: 14582205 Free PMC article.
Capped mRNAs with reduced secondary structure can function in extracts from poliovirus-infected cells.
Sonenberg N, Guertin D, Lee KA. Sonenberg N, et al. Mol Cell Biol. 1982 Dec;2(12):1633-8. doi: 10.1128/mcb.2.12.1633-1638.1982. Mol Cell Biol. 1982. PMID: 14582204 Free PMC article.
The translation initiation factor eIF-4E binds to a common motif shared by the translation factor eIF-4 gamma and the translational repressors 4E-binding proteins.
Mader S, Lee H, Pause A, Sonenberg N. Mader S, et al. Mol Cell Biol. 1995 Sep;15(9):4990-7. doi: 10.1128/MCB.15.9.4990. Mol Cell Biol. 1995. PMID: 7651417 Free PMC article.

References

1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. J Virol. 1971 Nov;8(5):661-8 - PubMed
1. Proc Natl Acad Sci U S A. 1976 Feb;73(2):327-30 - PubMed
1. Proc Natl Acad Sci U S A. 1976 Feb;73(2):375-80 - PubMed
1. J Virol. 1976 May;18(2):719-26 - PubMed

Purification of a factor that restores translation of vesicular stomatitis virus mRNA in extracts from poliovirus-infected HeLa cells - PubMed (original) (raw)