Assembly of the mitochondrial membrane system: isolation of mitochondrial transfer ribonucleic acid mutants and characterization of transfer ribonucleic acid genes of Saccharomyces cerevisiae - PubMed (original) (raw)

Assembly of the mitochondrial membrane system: isolation of mitochondrial transfer ribonucleic acid mutants and characterization of transfer ribonucleic acid genes of Saccharomyces cerevisiae

R E Berlani et al. J Bacteriol. 1980 Mar.

Abstract

A method is described for isolating cytoplasmic mutants of Saccharomyces cerevisiae with lesions in mitochondrial transfer ribonucleic acids (tRNA's). The mutants were selected for slow growth on glycerol and for restoration of wild-type growth by cytoplasmic "petite" testers that contain regions of mitochondrial deoxyribonucleic acid (DNA) with tRNA genes. The aminoacylated mitochondrial tRNA's of several presumptive tRNA mutants were analyzed by reverse-phase chromatography on RPC-5. Two mutant strains, G76-26 and G76-35, were determined to carry mutations in the cysteine and histidine tRNA genes, respectively. The cysteine tRNA mutant was used to isolate cytoplasmic petite mutants whose retained segments of mitochondrial DNA contain the cysteine tRNA gene. The segment of one such mutant (DS504) was sequenced and shown to have the cysteine, histidine, and threonine tRNA genes. The structures of the three mitochondrial tRNA's were deduced from the DNA sequence.

PubMed Disclaimer

Cited by

A mitochondrial frameshift suppressor maps in the tRNASer-var1 region of the mitochondrial genome of the yeast S. cerevisiae.
Weiss-Brummer B, Sakai H, Hüttenhofer A. Weiss-Brummer B, et al. Curr Genet. 1989 Apr;15(4):239-46. doi: 10.1007/BF00447038. Curr Genet. 1989. PMID: 2546683
Location of transcriptional control signals and transfer RNA sequences in Torulopsis glabrata mitochondrial DNA.
Clark-Walker GD, McArthur CR, Sriprakash KS. Clark-Walker GD, et al. EMBO J. 1985 Feb;4(2):465-73. doi: 10.1002/j.1460-2075.1985.tb03652.x. EMBO J. 1985. PMID: 4040462 Free PMC article.
Expression of the clustered mitochondrial tRNA genes in Saccharomyces cerevisiae: transcription and processing of transcripts.
Palleschi C, Francisci S, Zennaro E, Frontali L. Palleschi C, et al. EMBO J. 1984 Jun;3(6):1389-95. doi: 10.1002/j.1460-2075.1984.tb01982.x. EMBO J. 1984. PMID: 6086323 Free PMC article.
Aminoacylation and conformational properties of yeast mitochondrial tRNA mutants with respiratory deficiency.
Francisci S, DE Luca C, Oliva R, Morea V, Tramontano A, Frontali L. Francisci S, et al. RNA. 2005 Jun;11(6):914-27. doi: 10.1261/rna.2260305. RNA. 2005. PMID: 15923375 Free PMC article.
Organization and expression of a tRNA gene cluster in Saccharomyces cerevisiae mitochondrial DNA.
Miller DL, Martin NC. Miller DL, et al. Curr Genet. 1981 Nov;4(2):135-43. doi: 10.1007/BF00365692. Curr Genet. 1981. PMID: 24185959

References

1. Biochemistry. 1968 Feb;7(2):668-74 - PubMed
1. J Mol Biol. 1972 Feb 14;63(3):431-40 - PubMed
1. J Biol Chem. 1972 May 25;247(10):2989-3000 - PubMed
1. Eur J Biochem. 1973 Jan 15;32(2):301-11 - PubMed
1. Genetics. 1974 Feb;76(2):195-219 - PubMed

Assembly of the mitochondrial membrane system: isolation of mitochondrial transfer ribonucleic acid mutants and characterization of transfer ribonucleic acid genes of Saccharomyces cerevisiae - PubMed (original) (raw)