Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance - PubMed (original) (raw)
Partitioning of bacterial plasmids during cell division: a cis-acting locus that accomplishes stable plasmid inheritance
P A Meacock et al. Cell. 1980 Jun.
Abstract
We have identified and characterized a genetic function (designated par, for partition) that is required for stable maintenance of plasmids within exponentially growing cell populations. This function, which accomplishes the active distribution of plasmid DNA molecules to daughter cells, has been localized within the pSC101 plasmid to a 270 bp segment adjacent to the replication origin. The par locus, which appears to be functionally equivalent to the centromere of eucaryotic cells, is able to rescue unstable pSC101-derived replicons or an unrelated par- P15A-derived multicopy replicon in the cis, but not the trans, configuration. It is independent of copy number control and dose not specify plasmid incompatibility. Furthermore, it is not associated directly with plasmid replication functions.
Similar articles
- The partition (par) locus of pSC101 is an enhancer of plasmid incompatibility.
Miller CA, Cohen SN. Miller CA, et al. Mol Microbiol. 1993 Aug;9(4):695-702. doi: 10.1111/j.1365-2958.1993.tb01730.x. Mol Microbiol. 1993. PMID: 8231803 - Isolation and characterization of plasmid mutations that enable partitioning of pSC101 replicons lacking the partition (par) locus.
Conley DL, Cohen SN. Conley DL, et al. J Bacteriol. 1995 Feb;177(4):1086-9. doi: 10.1128/jb.177.4.1086-1089.1995. J Bacteriol. 1995. PMID: 7860588 Free PMC article. - Role of DNA superhelicity in partitioning of the pSC101 plasmid.
Miller CA, Beaucage SL, Cohen SN. Miller CA, et al. Cell. 1990 Jul 13;62(1):127-33. doi: 10.1016/0092-8674(90)90246-b. Cell. 1990. PMID: 2163765 - Aspects of plasmid F maintenance in Escherichia coli.
Kline BC. Kline BC. Can J Microbiol. 1988 Apr;34(4):526-35. doi: 10.1139/m88-090. Can J Microbiol. 1988. PMID: 3052759 Review. - Plasmid replication and partition in Escherichia coli: is the cell membrane the key?
Firshein W, Kim P. Firshein W, et al. Mol Microbiol. 1997 Jan;23(1):1-10. doi: 10.1046/j.1365-2958.1997.2061569.x. Mol Microbiol. 1997. PMID: 9004215 Review.
Cited by
- Single cell variability of CRISPR-Cas interference and adaptation.
McKenzie RE, Keizer EM, Vink JNA, van Lopik J, Büke F, Kalkman V, Fleck C, Tans SJ, Brouns SJJ. McKenzie RE, et al. Mol Syst Biol. 2022 Apr;18(4):e10680. doi: 10.15252/msb.202110680. Mol Syst Biol. 2022. PMID: 35467080 Free PMC article. - Np4A alarmones function in bacteria as precursors to RNA caps.
Luciano DJ, Belasco JG. Luciano DJ, et al. Proc Natl Acad Sci U S A. 2020 Feb 18;117(7):3560-3567. doi: 10.1073/pnas.1914229117. Epub 2020 Feb 4. Proc Natl Acad Sci U S A. 2020. PMID: 32019889 Free PMC article. - Stresses that Raise Np4A Levels Induce Protective Nucleoside Tetraphosphate Capping of Bacterial RNA.
Luciano DJ, Levenson-Palmer R, Belasco JG. Luciano DJ, et al. Mol Cell. 2019 Sep 5;75(5):957-966.e8. doi: 10.1016/j.molcel.2019.05.031. Epub 2019 Jun 6. Mol Cell. 2019. PMID: 31178354 Free PMC article. - Obstacles to Scanning by RNase E Govern Bacterial mRNA Lifetimes by Hindering Access to Distal Cleavage Sites.
Richards J, Belasco JG. Richards J, et al. Mol Cell. 2019 Apr 18;74(2):284-295.e5. doi: 10.1016/j.molcel.2019.01.044. Epub 2019 Mar 6. Mol Cell. 2019. PMID: 30852060 Free PMC article. - Localization of Low Copy Number Plasmid pRC4 in Replicating Rod and Non-Replicating Cocci Cells of Rhodococcus erythropolis PR4.
Singhi D, Jain A, Srivastava P. Singhi D, et al. PLoS One. 2016 Dec 9;11(12):e0166491. doi: 10.1371/journal.pone.0166491. eCollection 2016. PLoS One. 2016. PMID: 27935968 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources