Nucleotide sequence of Moloney leukemia virus: 3' end reveals details of replications, analogy to bacterial transposons, and an unexpected gene - PubMed (original) (raw)
Nucleotide sequence of Moloney leukemia virus: 3' end reveals details of replications, analogy to bacterial transposons, and an unexpected gene
J G Sutcliffe et al. Proc Natl Acad Sci U S A. 1980 Jun.
Abstract
We have determined the sequence of a cloned DNA fragment 1108 base pairs long which corresponds to the 3' end of the Moloney murine leukemia provirus. The clone was obtained as the primary product of reverse transcription and begins with the Moloney "strong stop" sequence, then extends towards the 5' end of the provirus. Our sequence: (i) proves that reverse transcriptase switches templates during minus strand synthesis; (ii) defines the limits of the 515-base-pair repeats which occupy both ends of the integrated provirus; (iii) shows that the structure of the proviral repeats has strong analogy to bacterial insertion sequences, indicating that the Moloney provirus is a transposon; (iv) identifies the putative promotor for genomic transcription within these repeats; (v) shows that the presumed origin of second strand synthesis, which lies just outside the 3' repeat, has tertiary structure analogous to single-stranded bacteriophage origins of replication; (vi) solves the amino acid sequence of most of pI5E, the carboxy-terminal product of the env gene; (vii) allows detailed mapping of the mink cell focus-forming virus substitution locus in a central location within the gp70 region of the env gene; and (viii) identifies a long open translation frame to the right of the env gene (R gene) which could be involved in leukemogenesis.
Similar articles
- Analysis of the env gene of a molecularly cloned and biologically active Moloney mink cell focus-forming proviral DNA.
Bosselman RA, van Straaten F, Van Beveren C, Verma IM, Vogt M. Bosselman RA, et al. J Virol. 1982 Oct;44(1):19-31. doi: 10.1128/JVI.44.1.19-31.1982. J Virol. 1982. PMID: 7143566 Free PMC article. - Structure of Moloney murine leukemia viral DNA: nucleotide sequence of the 5' long terminal repeat and adjacent cellular sequences.
Van Beveren C, Goddard JG, Berns A, Verma IM. Van Beveren C, et al. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3307-11. doi: 10.1073/pnas.77.6.3307. Proc Natl Acad Sci U S A. 1980. PMID: 6251455 Free PMC article. - Nucleotide sequences of integrated Moloney sarcoma provirus long terminal repeats and their host and viral junctions.
Dhar R, McClements WL, Enquist LW, Vande Woude GF. Dhar R, et al. Proc Natl Acad Sci U S A. 1980 Jul;77(7):3937-41. doi: 10.1073/pnas.77.7.3937. Proc Natl Acad Sci U S A. 1980. PMID: 6254003 Free PMC article. - Demonstration of biological activity and nucleotide sequence of an in vitro synthesized clone of the Moloney murine sarcoma virus mos gene.
Donoghue DJ. Donoghue DJ. J Virol. 1982 May;42(2):538-46. doi: 10.1128/JVI.42.2.538-546.1982. J Virol. 1982. PMID: 7045395 Free PMC article. - Identification of a RNA polymerase II initiation site in the long terminal repeat of Moloney murine leukemia viral DNA.
Fuhrman SA, Van Beveren C, Verma IM. Fuhrman SA, et al. Proc Natl Acad Sci U S A. 1981 Sep;78(9):5411-5. doi: 10.1073/pnas.78.9.5411. Proc Natl Acad Sci U S A. 1981. PMID: 6946480 Free PMC article.
Cited by
- How Well Does Evolution Explain Endogenous Retroviruses?-A Lakatosian Assessment.
Jorritsma RN. Jorritsma RN. Viruses. 2021 Dec 22;14(1):14. doi: 10.3390/v14010014. Viruses. 2021. PMID: 35062218 Free PMC article. Review. - Across the Hall from Pioneers.
Rein A. Rein A. Viruses. 2021 Mar 16;13(3):491. doi: 10.3390/v13030491. Viruses. 2021. PMID: 33809689 Free PMC article. - Transmission, Evolution, and Endogenization: Lessons Learned from Recent Retroviral Invasions.
Greenwood AD, Ishida Y, O'Brien SP, Roca AL, Eiden MV. Greenwood AD, et al. Microbiol Mol Biol Rev. 2017 Dec 13;82(1):e00044-17. doi: 10.1128/MMBR.00044-17. Print 2018 Mar. Microbiol Mol Biol Rev. 2017. PMID: 29237726 Free PMC article. Review. - Efficient insertion from an internal long terminal repeat (LTR)-LTR sequence on a reticuloendotheliosis virus vector is imprecise and cell specific.
Panganiban AT, Talbot KJ. Panganiban AT, et al. J Virol. 1993 Mar;67(3):1564-71. doi: 10.1128/JVI.67.3.1564-1571.1993. J Virol. 1993. PMID: 8382311 Free PMC article. - Analysis of the env gene of a molecularly cloned and biologically active Moloney mink cell focus-forming proviral DNA.
Bosselman RA, van Straaten F, Van Beveren C, Verma IM, Vogt M. Bosselman RA, et al. J Virol. 1982 Oct;44(1):19-31. doi: 10.1128/JVI.44.1.19-31.1982. J Virol. 1982. PMID: 7143566 Free PMC article.
References
- Proc Natl Acad Sci U S A. 1980 Jun;77(6):3307-11 - PubMed
- J Virol. 1978 Jun;26(3):615-29 - PubMed
- J Virol. 1976 Oct;20(1):324-9 - PubMed
- J Gen Virol. 1979 Jan;42(1):1-26 - PubMed
- J Virol. 1979 Feb;29(2):735-43 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources