Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement - PubMed (original) (raw)

Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement

L Bonen et al. Nucleic Acids Res. 1980.

Abstract

We show here that mitochondrial-specific ribosomal and transfer RNAs of wheat (Triticum vulgare Vill. [Triticum aestivum L.] var. Thatcher) are encoded by the mitochondrial DNA (mtDNA). Individual wheat mitochondrial rRNA species (26S, 18S, 5S) each hybridized with several mtDNA fragments in a particular restriction digest (Eco RI, Xho I, or Sal I). In each case, the DNA fragments to which 18S and 5S rRNAs hybridized were the same, but different from those to which 26S rRNA hybridized. From these results, we conclude that the structural genes for wheat mitochondrial 18S and 5S rRNAs are closely linked, but are physically distant from the genes for wheat mitochondrial 26S rRNA. This arrangement of rRNA genes is clearly different from that in prokaryotes and chloroplasts, where 23S, 16S and 5S rRNA genes are closely linked, even though wheat mitochondrial 18S rRNA has previously been shown to be prokaryotic in nature. The mixed population of wheat mitochondrial 4S RNAs (tRNAs) hybridized with many large restriction fragments, indicating that the tRNA genes are broadly distributed throughout the mitochondrial genome, with some apparent clustering in regions containing 18S and 5S rRNA genes.

PubMed Disclaimer

Cited by

Complete mitochondrial genome sequences of Brassica rapa (Chinese cabbage and mizuna), and intraspecific differentiation of cytoplasm in B. rapa and Brassica juncea.
Hatono S, Nishimura K, Murakami Y, Tsujimura M, Yamagishi H. Hatono S, et al. Breed Sci. 2017 Sep;67(4):357-362. doi: 10.1270/jsbbs.17023. Epub 2017 Jul 28. Breed Sci. 2017. PMID: 29085245 Free PMC article.
Mitochondrial genome sequences from wild and cultivated barley (Hordeum vulgare).
Hisano H, Tsujimura M, Yoshida H, Terachi T, Sato K. Hisano H, et al. BMC Genomics. 2016 Oct 24;17(1):824. doi: 10.1186/s12864-016-3159-3. BMC Genomics. 2016. PMID: 27776481 Free PMC article.
The complete mitochondrial genome sequence of Brassica oleracea and analysis of coexisting mitotypes.
Tanaka Y, Tsuda M, Yasumoto K, Terachi T, Yamagishi H. Tanaka Y, et al. Curr Genet. 2014 Nov;60(4):277-84. doi: 10.1007/s00294-014-0433-2. Epub 2014 Jun 12. Curr Genet. 2014. PMID: 24916859
Conservation of ribosomal RNA gene arrangement in the mitochondrial DNA of angiosperms.
Huh TY, Gray MW. Huh TY, et al. Plant Mol Biol. 1982 Sep;1(3):245-9. doi: 10.1007/BF00021035. Plant Mol Biol. 1982. PMID: 24317966
Genes for tRNA(Asp), tRNA (Pro), tRNA (Tyr) and two tRNAs (Ser) in wheat mitochondrial DNA.
Joyce PB, Spencer DF, Bonen L, Gray MW. Joyce PB, et al. Plant Mol Biol. 1988 May;10(3):251-62. doi: 10.1007/BF00027402. Plant Mol Biol. 1988. PMID: 24277519

References

1. Plant Physiol. 1974 May;53(5):677-83 - PubMed
1. Biochim Biophys Acta. 1976 Aug 2;442(1):24-31 - PubMed
1. Biochemistry. 1978 Jan 24;17(2):284-9 - PubMed
1. Biochim Biophys Acta. 1979 Mar 28;562(1):11-31 - PubMed
1. Biochimie. 1973;55(6):801-4 - PubMed

Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement - PubMed (original) (raw)