Transcripts of the adeno-associated virus genome: mapping of the major RNAs - PubMed (original) (raw)
Transcripts of the adeno-associated virus genome: mapping of the major RNAs
M R Green et al. J Virol. 1980 Oct.
Abstract
The four major adeno-associated virus type 2 (AAV2)-specific RNAs were mapped on the linear viral genome by a variety of biochemical techniques, including S1 nuclease and exonuclease VII mapping, RNA gel-transfer hybridization, and analysis of reverse transcriptase extension products. All the major AAV2 RNAs were derived from the minus DNA strand and had 3' termini at position 96. The nucleus-specific 4.3- and 3.6-kilobase (kb) RNAs had 5' termini at positions 6 and 19, respectively. The 5' terminus of the 2.6-kb RNA mapped to position 38.5. The predominant 2.3-kb AAV2 mRNA was spliced and contained a short leader sequence (approximately 50 nucleotides) which mapped to position 38.5, coincident with the 5' terminus of the 2.6-kb RNA. The 5' end of the body of the 2.3-kb RNA mapped to position 46.5. These results are discussed in terms of the involvement of single versus multiple promoters (for transcription) and RNA splicing mechanisms in the generation of the AAV2 RNAs.
Similar articles
- Mapping of the 5' termini of two adeno-associated virus 2 RNAs in the left half of the genome.
Lusby EW, Berns KI. Lusby EW, et al. J Virol. 1982 Feb;41(2):518-26. doi: 10.1128/JVI.41.2.518-526.1982. J Virol. 1982. PMID: 6281463 Free PMC article. - Transcripts of the adenovirus-associated virus genome: multiple polyadenylated RNAs including a potential primary transcript.
Green MR, Straus SE, Roeder RG. Green MR, et al. J Virol. 1980 Aug;35(2):560-5. doi: 10.1128/JVI.35.2.560-565.1980. J Virol. 1980. PMID: 6255194 Free PMC article. - Spliced adenovirus-associated virus RNA.
Laughlin CA, Westphal H, Carter BJ. Laughlin CA, et al. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5567-71. doi: 10.1073/pnas.76.11.5567. Proc Natl Acad Sci U S A. 1979. PMID: 230481 Free PMC article.
Cited by
- Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression.
Zhao Y, Liu W, Li Y, Ma J, Liu T, Cui H, Deng Y, Liao X, Wang Z. Zhao Y, et al. J Virol. 2024 Mar 19;98(3):e0151523. doi: 10.1128/jvi.01515-23. Epub 2024 Feb 7. J Virol. 2024. PMID: 38323812 Free PMC article. - Inducible HEK293 AAV packaging cell lines expressing Rep proteins.
Jalšić L, Lytvyn V, Elahi SM, Hrapovic S, Nassoury N, Chahal PS, Gaillet B, Gilbert R. Jalšić L, et al. Mol Ther Methods Clin Dev. 2023 Jul 15;30:259-275. doi: 10.1016/j.omtm.2023.07.002. eCollection 2023 Sep 14. Mol Ther Methods Clin Dev. 2023. PMID: 37560197 Free PMC article. - The Interplay between Adeno-Associated Virus and its Helper Viruses.
Meier AF, Fraefel C, Seyffert M. Meier AF, et al. Viruses. 2020 Jun 19;12(6):662. doi: 10.3390/v12060662. Viruses. 2020. PMID: 32575422 Free PMC article. Review. - Identification of a Functionally Relevant Adeno-Associated Virus Rep68 Oligomeric Interface.
Bardelli M, Zárate-Pérez F, Agúndez L, Linden RM, Escalante CR, Henckaerts E. Bardelli M, et al. J Virol. 2016 Jul 11;90(15):6612-6624. doi: 10.1128/JVI.00356-16. Print 2016 Aug 1. J Virol. 2016. PMID: 27170758 Free PMC article. - NP1 Protein of the Bocaparvovirus Minute Virus of Canines Controls Access to the Viral Capsid Genes via Its Role in RNA Processing.
Fasina OO, Dong Y, Pintel DJ. Fasina OO, et al. J Virol. 2015 Dec 4;90(4):1718-28. doi: 10.1128/JVI.02618-15. Print 2016 Feb 15. J Virol. 2015. PMID: 26637456 Free PMC article.
References
- J Virol. 1970 Jun;5(6):693-9 - PubMed
- Annu Rev Biochem. 1970;39:701-56 - PubMed
- Nat New Biol. 1973 Jul 18;244(133):71-3 - PubMed
- Anal Biochem. 1974 Mar;58(1):195-207 - PubMed
- J Mol Biol. 1976 Dec;108(2):491-511 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Medical