A small cosmid for efficient cloning of large DNA fragments - PubMed (original) (raw)
A small cosmid for efficient cloning of large DNA fragments
B Hohn et al. Gene. 1980 Nov.
Abstract
The production and use of the 6 kb cosmid pHC79, a derivative of pBR322, is described. It can be used for cloning of fragments cleaved by EcoRI, ClaI, BamHI (also BglII, BclI, Sau3A and MboI), SalI (also XhoI and AvaI), EcaI and PstI. Hybrid cosmids containing inserts in the size range of 40 kb are packaged in vitro and transduced with an efficiency of 5 X 10(4) - 5 X 10(5) clones/microgram of insert DNA. Prefractionation of the DNA fragments to be cloned into 40 kb sized fragments ensures the cloning of contiguous stretches of DNA. Proteins produced in vitro by the cosmid pHC79 are identical to the ones produced by its pBR322 parent.
Similar articles
- Efficient simplified cosmid cloning: construction and characterization of cosmid vectors that carry the two cohesive end sites of lambda phages arrayed in tandem.
Ishiura M, Ohashi H, Uchida T, Okada Y. Ishiura M, et al. Anal Biochem. 1989 Jan;176(1):117-27. doi: 10.1016/0003-2697(89)90281-9. Anal Biochem. 1989. PMID: 2523674 - A bacteriophage lambda vector for cloning large DNA fragments made with several restriction enzymes.
Loenen WA, Brammar WJ. Loenen WA, et al. Gene. 1980 Aug;10(3):249-59. doi: 10.1016/0378-1119(80)90054-2. Gene. 1980. PMID: 6254843 - New cosmid vectors developed for eukaryotic DNA cloning.
Brady G, Jantzen HM, Bernard HU, Brown R, Schütz G, Hashimoto-Gotoh T. Brady G, et al. Gene. 1984 Feb;27(2):223-32. doi: 10.1016/0378-1119(84)90143-4. Gene. 1984. PMID: 6327467 - [Manipulation of embryos, cells, nuclei and genes. II. Genetic engineering].
Zorzopulos J, Denoya CD. Zorzopulos J, et al. Rev Argent Microbiol. 1982 Jan-Mar;14(1):41-76. Rev Argent Microbiol. 1982. PMID: 6100999 Review. Spanish. No abstract available. - Bacteriophage lambda as a cloning vector.
Chauthaiwale VM, Therwath A, Deshpande VV. Chauthaiwale VM, et al. Microbiol Rev. 1992 Dec;56(4):577-91. doi: 10.1128/mr.56.4.577-591.1992. Microbiol Rev. 1992. PMID: 1480110 Free PMC article. Review.
Cited by
- Microfluidic automated plasmid library enrichment for biosynthetic gene cluster discovery.
Xu P, Modavi C, Demaree B, Twigg F, Liang B, Sun C, Zhang W, Abate AR. Xu P, et al. Nucleic Acids Res. 2020 May 7;48(8):e48. doi: 10.1093/nar/gkaa131. Nucleic Acids Res. 2020. PMID: 32095820 Free PMC article. - The art of vector engineering: towards the construction of next-generation genetic tools.
Nora LC, Westmann CA, Martins-Santana L, Alves LF, Monteiro LMO, Guazzaroni ME, Silva-Rocha R. Nora LC, et al. Microb Biotechnol. 2019 Jan;12(1):125-147. doi: 10.1111/1751-7915.13318. Epub 2018 Sep 26. Microb Biotechnol. 2019. PMID: 30259693 Free PMC article. Review. - The Ferric uptake regulator (Fur) and iron availability control the production and maturation of the antibacterial peptide microcin E492.
Marcoleta AE, Gutiérrez-Cortez S, Hurtado F, Argandoña Y, Corsini G, Monasterio O, Lagos R. Marcoleta AE, et al. PLoS One. 2018 Aug 2;13(8):e0200835. doi: 10.1371/journal.pone.0200835. eCollection 2018. PLoS One. 2018. PMID: 30071030 Free PMC article. - Identification of Key Amino Acid Residues Modulating Intracellular and In vitro Microcin E492 Amyloid Formation.
Aguilera P, Marcoleta A, Lobos-Ruiz P, Arranz R, Valpuesta JM, Monasterio O, Lagos R. Aguilera P, et al. Front Microbiol. 2016 Jan 28;7:35. doi: 10.3389/fmicb.2016.00035. eCollection 2016. Front Microbiol. 2016. PMID: 26858708 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Other Literature Sources
Research Materials