Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein - PubMed (original) (raw)

. 1981 Aug 13;292(5824):595-600.

doi: 10.1038/292595a0.

• PMID: 6265803
• DOI: 10.1038/292595a0

Transformation of rat cells by an altered polyoma virus genome expressing only the middle-T protein

R Treisman et al. Nature. 1981.

Abstract

A modified polyoma virus genome has been constructed which can encode the middle-T protein, but not the large-T or small-T proteins. This was achieved, starting with the full length viral DNA inserted into a plasmid vector, by replacing a small genomic restriction fragment spanning the middle-T intervening sequence with the equivalent fragment from a cloned partial cDNA copy of the middle-T protein mRNA. Transfection of the modified viral DNA into cultured rat cells efficiently induced the formation of transformed cell foci which gave rise to cell lines that grew as tumours after injection into Fisher rats. The only viral early-region antigen synthesized by the cell lines was the middle-T protein. Expression of the middle-t protein is therefore sufficient to establish and maintain a transformed state. The viral mRNA produced by two of the transformed cell lines was structurally indistinguishable from the normal middle-T mRNA found in productively infected cells, suggesting that RNA splicing is not an essential step in the biogenesis of this messenger.

PubMed Disclaimer

Similar articles

• Coding capacity of a 35 percent fragment of the polyoma virus genome is sufficient to initiate and maintain cellular transformation.
  Novak U, Dilworth SM, Griffin BE. Novak U, et al. Proc Natl Acad Sci U S A. 1980 Jun;77(6):3278-82. doi: 10.1073/pnas.77.6.3278. Proc Natl Acad Sci U S A. 1980. PMID: 6251451 Free PMC article.
• Middle T antigen as primary inducer of full expression of the phenotype of transformation by polyoma virus.
  Ito Y, Spurr N, Griffin BE. Ito Y, et al. J Virol. 1980 Jul;35(1):219-32. doi: 10.1128/JVI.35.1.219-232.1980. J Virol. 1980. PMID: 6251270 Free PMC article.
• Transformation of rat fibroblasts by cloned defective polyoma DNA.
  Parent C, Bastin M. Parent C, et al. Arch Int Physiol Biochim. 1981 Sep;89(3):225-33. doi: 10.3109/13813458109069470. Arch Int Physiol Biochim. 1981. PMID: 6171226
• The tumor antigens and the early functions of polyoma virus.
  Türler H. Türler H. Mol Cell Biochem. 1980 Sep 15;32(2):63-93. doi: 10.1007/BF00227801. Mol Cell Biochem. 1980. PMID: 6256621 Review. No abstract available.
• Functions of T antigens of SV40 and polyomavirus.
  Hand R. Hand R. Biochim Biophys Acta. 1981 Aug 31;651(1):1-24. doi: 10.1016/0304-419x(81)90002-0. Biochim Biophys Acta. 1981. PMID: 6169368 Review. No abstract available.

Cited by

• Membrane-bound Merkel cell polyomavirus middle T protein constitutively activates PLCγ1 signaling through Src-family kinases.
  Peng WY, Abere B, Shi H, Toland S, Smithgall TE, Moore PS, Chang Y. Peng WY, et al. Proc Natl Acad Sci U S A. 2023 Dec 19;120(51):e2316467120. doi: 10.1073/pnas.2316467120. Epub 2023 Dec 11. Proc Natl Acad Sci U S A. 2023. PMID: 38079542 Free PMC article.
• Polyomavirus Wakes Up and Chooses Neurovirulence.
  Butic AB, Spencer SA, Shaheen SK, Lukacher AE. Butic AB, et al. Viruses. 2023 Oct 18;15(10):2112. doi: 10.3390/v15102112. Viruses. 2023. PMID: 37896889 Free PMC article. Review.
• Insights from transgenic mouse models of PyMT-induced breast cancer: recapitulating human breast cancer progression in vivo.
  Attalla S, Taifour T, Bui T, Muller W. Attalla S, et al. Oncogene. 2021 Jan;40(3):475-491. doi: 10.1038/s41388-020-01560-0. Epub 2020 Nov 24. Oncogene. 2021. PMID: 33235291 Free PMC article. Review.
• Discovery of the PTEN Tumor Suppressor and Its Connection to the PI3K and AKT Oncogenes.
  Parsons R. Parsons R. Cold Spring Harb Perspect Med. 2020 Aug 3;10(8):a036129. doi: 10.1101/cshperspect.a036129. Cold Spring Harb Perspect Med. 2020. PMID: 31932465 Free PMC article. Review.
• Small size, big impact: how studies of small DNA tumour viruses revolutionized biology.
  DiMaio D. DiMaio D. Philos Trans R Soc Lond B Biol Sci. 2019 May 27;374(1773):20180300. doi: 10.1098/rstb.2018.0300. Philos Trans R Soc Lond B Biol Sci. 2019. PMID: 30955494 Free PMC article. Review.

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Nature Publishing Group

• Other Literature Sources

  • The Lens - Patent Citations Database