Radioimmunochemical measurement of the transferrin receptor in human trophoblast and reticulocyte membranes with a specific anti-receptor antibody - PubMed (original) (raw)

Radioimmunochemical measurement of the transferrin receptor in human trophoblast and reticulocyte membranes with a specific anti-receptor antibody

C A Enns et al. Proc Natl Acad Sci U S A. 1981 Jul.

Abstract

A radioimmunoassay was developed to directly assay the presence of transferrin receptors in human tissues. Antisera developed in a goat against purified human placental transferrin binding protein was purified by fractional sodium sulfate precipitation and adsorption against Sepharose-bound transferrin to remove trace anti-transferrin activity. The antisera immunoprecipitates a Mr 94,000 peptide on 125I-iodinated syncytial trophoblast membranes from placentae. This polypeptide has been identified previously as the transferrin binding protein of the placenta [Wada, H. G., Hass, P. E. & Sussman, H. H. (1979) J. Biol. Chem. 254, 12629-12635]. A standard curve using purified 125I-iodinated placental transferrin receptor and various amounts of the purified noniodinated receptor is sensitive from 5 to 900 ng. A reticulocyte-enriched membrane ghost preparation (5% reticulocyte) gives a value of 9.5 micrograms of receptor per mg of protein. Normal erythrocyte membrane ghosts show binding (0.57 micrograms of receptor per mg of protein) proportional to the amount of reticulocytes normally present in blood (0.5-1.0%). In other tissues in which the transferrin receptor binding has been reported, purified syncytial trophoblastic membranes are found to have 34.5 micrograms of receptor per mg of protein, and BeWo cells, a choriocarcinoma cell line, are found to have 15.7 micrograms of receptor per mg of protein. In contrast, normal breast tissue, which has no demonstrated transferrin binding, contains only 0.18 micrograms of receptor per mg of protein by this method.

PubMed Disclaimer

Cited by

Delayed hepcidin response explains the lag period in iron absorption following a stimulus to increase erythropoiesis.
Frazer DM, Inglis HR, Wilkins SJ, Millard KN, Steele TM, McLaren GD, McKie AT, Vulpe CD, Anderson GJ. Frazer DM, et al. Gut. 2004 Oct;53(10):1509-15. doi: 10.1136/gut.2003.037416. Gut. 2004. PMID: 15361505 Free PMC article.
Transferrin receptor is inversely correlated with estrogen receptor in breast cancer.
Tonik SE, Shindelman JE, Sussman HH. Tonik SE, et al. Breast Cancer Res Treat. 1986;7(2):71-6. doi: 10.1007/BF01806791. Breast Cancer Res Treat. 1986. PMID: 3013349
Transferrin receptors of human fibroblasts. Analysis of receptor properties and regulation.
Ward JH, Kushner JP, Kaplan J. Ward JH, et al. Biochem J. 1982 Oct 15;208(1):19-26. doi: 10.1042/bj2080019. Biochem J. 1982. PMID: 6297460 Free PMC article.
Studies of the transferrin receptor on both human reticulocytes and nucleated human cells in culture: comparison of factors regulating receptor density.
Frazier JL, Caskey JH, Yoffe M, Seligman PA. Frazier JL, et al. J Clin Invest. 1982 Apr;69(4):853-65. doi: 10.1172/jci110525. J Clin Invest. 1982. PMID: 6804495 Free PMC article.
Apical and basolateral transferrin receptors in polarized BeWo cells recycle through separate endosomes.
Cerneus DP, van der Ende A. Cerneus DP, et al. J Cell Biol. 1991 Sep;114(6):1149-58. doi: 10.1083/jcb.114.6.1149. J Cell Biol. 1991. PMID: 1910050 Free PMC article.

References

1. Biochim Biophys Acta. 1971 Dec 28;251(3):363-9 - PubMed
1. Cancer Res. 1968 Jul;28(7):1231-6 - PubMed
1. Biochemistry. 1969 Mar;8(3):1133-41 - PubMed
1. Nature. 1970 Aug 15;227(5259):680-5 - PubMed
1. Anal Biochem. 1975 Feb;63(2):414-7 - PubMed

Radioimmunochemical measurement of the transferrin receptor in human trophoblast and reticulocyte membranes with a specific anti-receptor antibody - PubMed (original) (raw)