The hydrolysis of phosphatidylinositol monolayers at an air/water interface by the calcium-ion-dependent phosphatidylinositol phosphodiesterase of pig brain - PubMed (original) (raw)

The hydrolysis of phosphatidylinositol monolayers at an air/water interface by the calcium-ion-dependent phosphatidylinositol phosphodiesterase of pig brain

K Hirasawa et al. Biochem J. 1981.

Abstract

1. The activity of Ca2+-dependent phosphatidylinositol phosphodiesterase (EC 3.1.4.10) of pig brain against [32P]phosphatidylinositol monolayers at an air/water interface has been measured. As the monolayer pressure was increased a sharp cut-off of enzymic hydrolysis occurred at 33 X 10(-3) N/m. 2. The addition of either phosphatidic acid, phosphatidylglycerol or oleyl alcohol increased the film pressure at which cut off occurred, as well as increasing the rate of hydrolysis at lower pressures. 3. The rate of hydrolysis, but not the cut-off pressure, was markedly increased by oleic acid and slightly increased by phosphatidylethanolamine. 4. Phosphatidylcholine, palmitoylcholine and octadecylamine decreased the cut-off pressure, as well as the enzymic activity below this pressure. 5. Stearic acid and stearyl alcohol had no effect on either the cut-off pressure or the activity. 6. All activators decreased the length of the lag phase before enzyme activity began, and phosphatidylcholine increased it. 7. These results are compared with the stimulatory and inhibitory effects of various amphiphiles observed previously with phosphatidylinositol dispersions [Irvine, Hemington & Dawson (1979) Eur. J. Biochem. 99, 525-530], and their possible relevance to the control of the phosphatidylinositol phosphodiesterase in vivo are discussed.

PubMed Disclaimer

Similar articles

• The calcium-dependent phosphatidylinositol-phosphodiesterase of rat brain. Mechanisms of suppression and stimulation.
  Irvine RF, Hemington N, Dawson RM. Irvine RF, et al. Eur J Biochem. 1979 Sep;99(3):525-30. doi: 10.1111/j.1432-1033.1979.tb13284.x. Eur J Biochem. 1979. PMID: 227685
• Phosphatidylinositol phosphodiesterase in higher plants.
  Irvine RF, Letcher AJ, Dawson RM. Irvine RF, et al. Biochem J. 1980 Oct 15;192(1):279-83. doi: 10.1042/bj1920279. Biochem J. 1980. PMID: 6272700 Free PMC article.
• Fatty acid stimulation of membrane phosphatidylinositol hydrolysis by brain phosphatidylinositol phosphodiesterase.
  Irvine RF, Letcher AJ, Dawson RM. Irvine RF, et al. Biochem J. 1979 Feb 15;178(2):497-500. doi: 10.1042/bj1780497. Biochem J. 1979. PMID: 220968 Free PMC article.
• The inhibition and activation of Ca2+-dependent phosphatidylinositol phosphodiesterase by phospholipids and blood plasma.
  Dawson RM, Hemington N, Irvine RF. Dawson RM, et al. Eur J Biochem. 1980 Nov;112(1):33-8. doi: 10.1111/j.1432-1033.1980.tb04983.x. Eur J Biochem. 1980. PMID: 6256168

Cited by

• Interfacial hydrolysis of phosphatidylinositol 4-phosphate and phosphatidylinositol 4,5-bisphosphate by turkey erythrocyte phospholipase C.
  James SR, Demel RA, Downes CP. James SR, et al. Biochem J. 1994 Mar 1;298 ( Pt 2)(Pt 2):499-506. doi: 10.1042/bj2980499. Biochem J. 1994. PMID: 8135761 Free PMC article.
• Inositol 1,2-cyclic 4,5-trisphosphate is not a product of muscarinic receptor-stimulated phosphatidylinositol 4,5-bisphosphate hydrolysis in rat parotid glands.
  Hawkins PT, Berrie CP, Morris AJ, Downes CP. Hawkins PT, et al. Biochem J. 1987 Apr 1;243(1):211-8. doi: 10.1042/bj2430211. Biochem J. 1987. PMID: 3038079 Free PMC article.
• Phosphatidic acid and phosphatidylinositol labelling in adipose tissue. Relationship to the metabolic effects of insulin and insulin-like agents.
  Honeyman TW, Strohsnitter W, Scheid CR, Schimmel RJ. Honeyman TW, et al. Biochem J. 1983 May 15;212(2):489-98. doi: 10.1042/bj2120489. Biochem J. 1983. PMID: 6411068 Free PMC article.
• Biophysical methods for the characterization of PTEN/lipid bilayer interactions.
  Harishchandra RK, Neumann BM, Gericke A, Ross AH. Harishchandra RK, et al. Methods. 2015 May;77-78:125-35. doi: 10.1016/j.ymeth.2015.02.004. Epub 2015 Feb 16. Methods. 2015. PMID: 25697761 Free PMC article. Review.
• A physicochemical study of the interaction of phosphatidylinositol with buprenorphine and naloxone.
  Reig F, Espígol C, García Antón JM, Valencia G, Alsina MA. Reig F, et al. J Bioenerg Biomembr. 1988 Aug;20(4):533-49. doi: 10.1007/BF00762208. J Bioenerg Biomembr. 1988. PMID: 3220837

References

1. 1. Biochem J. 1967 Jan;102(1):76-86 - PubMed
2. 1. Biochem Soc Trans. 1980 Feb;8(1):27-30 - PubMed
3. 1. Biochim Biophys Acta. 1962 May 7;59:103-15 - PubMed
4. 1. Biochem J. 1963 Sep;88:414-23 - PubMed
5. 1. Biochem J. 1960 Apr;75:133-8 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Europe PubMed Central
  • PubMed Central
  • Silverchair Information Systems

• Research Materials

  • NCI CPTC Antibody Characterization Program

• Miscellaneous

  • NCI CPTAC Assay Portal