Association of microtubules and intermediate filaments in normal fibroblasts and its disruption upon transformation by a temperature-sensitive mutant of Rous sarcoma virus - PubMed (original) (raw)

Association of microtubules and intermediate filaments in normal fibroblasts and its disruption upon transformation by a temperature-sensitive mutant of Rous sarcoma virus

E H Ball et al. Proc Natl Acad Sci U S A. 1981 Nov.

Abstract

By double indirect immunofluorescence, using primary rabbit antibodies to tubulin and guinea pig antibodies to vimentin, we have simultaneously labeled microtubules and intermediate filaments in several types of cultured normal fibroblasts. With well-spread interphase cells there was an extensive but not complete correspondence of the labeling patterns for the two filamentous structures out to the cell periphery. This correspondence existed both at a gross level, where parallel but not coincident arrays of thickly labeled strands of the two types of filaments were observed, and at a fine level, where thinly labeled strands of the two were superimposed. The results suggest that there may be some type(s) of molecular linkages between microtubules and vimentin intermediate filaments that is under metabolic control. With NRK fibroblasts infected with a temperature-sensitive mutant (LA23) of Rous sarcoma virus, cells grown at the nonpermissive temperature (39 degrees C) showed the correspondence of the distributions of the microtubules and intermediate filaments characteristic of the normal phenotype but within 1 hr after a shift to the permissive temperature (33 degrees C) there was an extensive retraction of the intermediate filaments around the cell nucleus whereas the microtubules remained dispersed into the cell periphery. These results suggest that one of the functions carried out by p60src, the protein kinase responsible for transformation by Rous sarcoma virus, may be to modify the component(s) involved in the putative linkages between microtubules and intermediate filaments in the normal cells.

PubMed Disclaimer

Similar articles

• Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts.
  Ball EH, Singer SJ. Ball EH, et al. Proc Natl Acad Sci U S A. 1982 Jan;79(1):123-6. doi: 10.1073/pnas.79.1.123. Proc Natl Acad Sci U S A. 1982. PMID: 6275382 Free PMC article.
• Immunofluorescence on avian sarcoma virus-transformed cells: localization of the src gene product.
  Rohrschneider LR. Rohrschneider LR. Cell. 1979 Jan;16(1):11-24. doi: 10.1016/0092-8674(79)90183-1. Cell. 1979. PMID: 217542
• Temperature-sensitive changes in surface modulating assemblies of fibroblasts transformed by mutants of Rous sarcoma virus.
  Edelman GM, Yahara I. Edelman GM, et al. Proc Natl Acad Sci U S A. 1976 Jun;73(6):2047-51. doi: 10.1073/pnas.73.6.2047. Proc Natl Acad Sci U S A. 1976. PMID: 180536 Free PMC article.
• The "Third Violin" in the Cytoskeleton Orchestra-The Role of Intermediate Filaments in the Endothelial Cell's Life.
  Shakhov AS, Alieva IB. Shakhov AS, et al. Biomedicines. 2022 Apr 1;10(4):828. doi: 10.3390/biomedicines10040828. Biomedicines. 2022. PMID: 35453578 Free PMC article. Review.
• The cytoskeleton of the hepatocyte. Structure and functions.
  Feldmann G. Feldmann G. J Hepatol. 1989 May;8(3):380-6. doi: 10.1016/0168-8278(89)90038-x. J Hepatol. 1989. PMID: 2659657 Review. No abstract available.

Cited by

• Scaling up single-cell mechanics to multicellular tissues - the role of the intermediate filament-desmosome network.
  Broussard JA, Jaiganesh A, Zarkoob H, Conway DE, Dunn AR, Espinosa HD, Janmey PA, Green KJ. Broussard JA, et al. J Cell Sci. 2020 Mar 16;133(6):jcs228031. doi: 10.1242/jcs.228031. J Cell Sci. 2020. PMID: 32179593 Free PMC article. Review.
• Microtubules: Evolving roles and critical cellular interactions.
  Logan CM, Menko AS. Logan CM, et al. Exp Biol Med (Maywood). 2019 Nov;244(15):1240-1254. doi: 10.1177/1535370219867296. Epub 2019 Aug 6. Exp Biol Med (Maywood). 2019. PMID: 31387376 Free PMC article. Review.
• Vimentin on the move: new developments in cell migration.
  Battaglia RA, Delic S, Herrmann H, Snider NT. Battaglia RA, et al. F1000Res. 2018 Nov 15;7:F1000 Faculty Rev-1796. doi: 10.12688/f1000research.15967.1. eCollection 2018. F1000Res. 2018. PMID: 30505430 Free PMC article. Review.
• Microtubule-dependent transport and dynamics of vimentin intermediate filaments.
  Hookway C, Ding L, Davidson MW, Rappoport JZ, Danuser G, Gelfand VI. Hookway C, et al. Mol Biol Cell. 2015 May 1;26(9):1675-86. doi: 10.1091/mbc.E14-09-1398. Epub 2015 Feb 25. Mol Biol Cell. 2015. PMID: 25717187 Free PMC article.
• Renal cells express different forms of vimentin: the independent expression alteration of these forms is important in cell resistance to osmotic stress and apoptosis.
  Buchmaier BS, Bibi A, Müller GA, Dihazi GH, Eltoweissy M, Kruegel J, Dihazi H. Buchmaier BS, et al. PLoS One. 2013 Jul 11;8(7):e68301. doi: 10.1371/journal.pone.0068301. Print 2013. PLoS One. 2013. PMID: 23874579 Free PMC article.

References

1. 1. J Cell Biol. 1968 Sep;38(3):538-55 - PubMed
2. 1. Cell. 1981 Apr;24(1):185-93 - PubMed
3. 1. J Cell Biol. 1971 Dec;51(3):752-62 - PubMed
4. 1. Proc Natl Acad Sci U S A. 1977 Sep;74(9):3883-7 - PubMed
5. 1. Cell. 1978 Jan;13(1):151-63 - PubMed

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Atypon
  • Europe PubMed Central
  • PubMed Central