Adenine nucleotide and lysine transport in Chlamydia psittaci - PubMed (original) (raw)
Adenine nucleotide and lysine transport in Chlamydia psittaci
T P Hatch et al. J Bacteriol. 1982 May.
Abstract
Isolated reticulate bodies of Chlamydia psittaci were found to transport ATP and ADP by an ATP-ADP exchange mechanism. ATP uptake activity was not detected in elementary bodies. The apparent Km of transport for both ATP and ADP was approximately 5 microM, and the calculated Vmax for both was about 1 nmol of nucleotide transported per min per mg of protein. ADP competitively inhibited ATP transport with a Ki of 4.5 microM. Other nucleotides tested had no effect on the uptake of ATP. A magnesium-dependent, oligomycin-sensitive ATPase (ATP phosphohydrolase, EC 3.6.1.3) was associated with reticulate bodies, and most of the transported ATP was hydrolyzed to ADP, which was exchanged for additional, extracellular nucleotide. Some ADP was hydrolyzed to AMP, which exited the cells slowly. Lysine was transported against the electrochemical gradient by reticulate bodies in the presence of ATP. Oligomycin and carbonyl cyanide p-trifluoromethoxyphenylhydrazone inhibited ATP-dependent lysine transport. Lysine exited reticulate bodies when the reticulate bodies were incubated in the presence of ADP, carbonyl cyanide p-trifluoromethoxyphenylhydrazone, or a reduced concentration of ATP. The results support the concept that chlamydiae are energy parasites which are capable of drawing upon the adenine nucleotides of their hosts, hydrolyzing ATP, and establishing an energized membrane.
Similar articles
- Synthesis of protein in host-free reticulate bodies of Chlamydia psittaci and Chlamydia trachomatis.
Hatch TP, Miceli M, Silverman JA. Hatch TP, et al. J Bacteriol. 1985 Jun;162(3):938-42. doi: 10.1128/jb.162.3.938-942.1985. J Bacteriol. 1985. PMID: 3997784 Free PMC article. - Transport of AMP by Rickettsia prowazekii.
Atkinson WH, Winkler HH. Atkinson WH, et al. J Bacteriol. 1985 Jan;161(1):32-8. doi: 10.1128/jb.161.1.32-38.1985. J Bacteriol. 1985. PMID: 3918004 Free PMC article. - Rickettsial permeability. An ADP-ATP transport system.
Winkler HH. Winkler HH. J Biol Chem. 1976 Jan 25;251(2):389-96. J Biol Chem. 1976. PMID: 1389 - Adenine nucleotide transport in sonic submitochondrial particles. Kinetic properties and binding of specific inhibitors.
Lauquin GJ, Villiers C, Michejda JW, Hryniewiecka LV, Vignais PV. Lauquin GJ, et al. Biochim Biophys Acta. 1977 May 11;460(2):331-45. doi: 10.1016/0005-2728(77)90219-5. Biochim Biophys Acta. 1977. PMID: 15594 - Molecular and physiological aspects of adenine nucleotide transport in mitochondria.
Vignais PV. Vignais PV. Biochim Biophys Acta. 1976 Apr 30;456(1):1-38. doi: 10.1016/0304-4173(76)90007-0. Biochim Biophys Acta. 1976. PMID: 131583 Review. No abstract available.
Cited by
- Chlamydia Infection Remodels Host Cell Mitochondria to Alter Energy Metabolism and Subvert Apoptosis.
Cheong HC, Sulaiman S, Looi CY, Chang LY, Wong WF. Cheong HC, et al. Microorganisms. 2023 May 24;11(6):1382. doi: 10.3390/microorganisms11061382. Microorganisms. 2023. PMID: 37374883 Free PMC article. Review. - Host and Bacterial Glycolysis during Chlamydia trachomatis Infection.
Ende RJ, Derré I. Ende RJ, et al. Infect Immun. 2020 Nov 16;88(12):e00545-20. doi: 10.1128/IAI.00545-20. Print 2020 Nov 16. Infect Immun. 2020. PMID: 32900818 Free PMC article. - Host cell death during infection with Chlamydia: a double-edged sword.
Sixt BS. Sixt BS. FEMS Microbiol Rev. 2021 Jan 8;45(1):fuaa043. doi: 10.1093/femsre/fuaa043. FEMS Microbiol Rev. 2021. PMID: 32897321 Free PMC article. Review. - Interaction of different Chlamydiae species with bovine spermatozoa.
Eckert T, Goericke-Pesch S, Heydel C, Bergmann M, Kauffold J, Failing K, Wehrend A. Eckert T, et al. BMC Microbiol. 2019 Jan 25;19(1):23. doi: 10.1186/s12866-019-1392-z. BMC Microbiol. 2019. PMID: 30683062 Free PMC article. - Therapeutic Targets in Chlamydial Fatty Acid and Phospholipid Synthesis.
Yao J, Rock CO. Yao J, et al. Front Microbiol. 2018 Sep 25;9:2291. doi: 10.3389/fmicb.2018.02291. eCollection 2018. Front Microbiol. 2018. PMID: 30319589 Free PMC article. Review.
References
- Biochim Biophys Acta. 1965 Jun 15;104(1):312-5 - PubMed
- Biochim Biophys Acta. 1965 Aug 24;107(1):184-8 - PubMed
- J Bacteriol. 1967 Oct;94(4):867-9 - PubMed
- J Biol Chem. 1968 Jan 10;243(1):20-8 - PubMed
- Biochemistry. 1968 Nov;7(11):4030-4 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials