Differential expression of the members of the discoidin I multigene family during growth and development of Dictyostelium discoideum - PubMed (original) (raw)
Differential expression of the members of the discoidin I multigene family during growth and development of Dictyostelium discoideum
J M Devine et al. Cell. 1982 Apr.
Abstract
Discoidin I and II are lectins synthesized during the aggregation of Dictyostelium discoideum amoebae which may play a role in cellular cohesion. Discoidin I was thought to consist of two major polypeptides, but we show that there are three. The N-terminal amino acid sequence of the polypeptides has been predicted by determining part of the nucleotide sequence of their respective mRNAs. We obtained the nucleotide sequences by reverse transcription of the mRNAs, using as primers, fragments derived from the coding region of two cloned discoidin I sequences, and utilizing cross hybridization to the various mRNA species and differences in the length of their 5' noncoding regions to isolate fragments for DNA sequencing. We used primer extension to measure the relative concentration of the three major discoidin I mRNA sequences. We show that during development changes in the abundance of all three mRNA sequences occur coordinately. In cells growing in nutrient broth, however, only two of the three major discoidin I mRNA sequences accumulate, and if such cells are grown to a very high density, both sequences disappear. These results indicate that the coordination of discoidin I gene expression is not obligatory and that the members of this multigene family may differ in the mode of their induction during normal development.
Similar articles
- Analysis of the multigene family coding the developmentally regulated carbohydrate-binding protein discoidin-I in D. discoideum.
Rowekamp W, Poole S, Firtel RA. Rowekamp W, et al. Cell. 1980 Jun;20(2):495-505. doi: 10.1016/0092-8674(80)90636-4. Cell. 1980. PMID: 7388948 - Characterization of sequence elements at the 5' end of a discoidin I gene isolated from Dictyostelium discoideum.
Devine JM, Williams JG. Devine JM, et al. Nucleic Acids Res. 1982 Feb 25;10(4):1231-41. doi: 10.1093/nar/10.4.1231. Nucleic Acids Res. 1982. PMID: 6280135 Free PMC article. - Molecular cloning and characterization of the cDNA for discoidin II of Dictyostelium discoideum.
Fukuzawa M, Ochiai H. Fukuzawa M, et al. Plant Cell Physiol. 1996 Jun;37(4):505-14. doi: 10.1093/oxfordjournals.pcp.a028973. Plant Cell Physiol. 1996. PMID: 8759914 - Expression of recombinant glycoproteins in the simple eukaryote Dictyostelium discoideum.
Slade MB, Emslie KR, Williams KL. Slade MB, et al. Biotechnol Genet Eng Rev. 1997;14:1-35. doi: 10.1080/02648725.1997.10647937. Biotechnol Genet Eng Rev. 1997. PMID: 9188148 Review. No abstract available. - Slime mold lectins.
Bartles JR, Frazier WA, Rosen SD. Bartles JR, et al. Int Rev Cytol. 1982;75:61-99. doi: 10.1016/s0074-7696(08)61002-5. Int Rev Cytol. 1982. PMID: 7049989 Review. No abstract available.
Cited by
- Transcriptional pulsing of a developmental gene.
Chubb JR, Trcek T, Shenoy SM, Singer RH. Chubb JR, et al. Curr Biol. 2006 May 23;16(10):1018-25. doi: 10.1016/j.cub.2006.03.092. Curr Biol. 2006. PMID: 16713960 Free PMC article. - Specific cell-cell contact serves as the developmental signal to deactivate discoidin I gene expression in Dictyostelium discoideum.
Berger EA, Clark JM. Berger EA, et al. Proc Natl Acad Sci U S A. 1983 Aug;80(16):4983-7. doi: 10.1073/pnas.80.16.4983. Proc Natl Acad Sci U S A. 1983. PMID: 16593353 Free PMC article. - Identification of a DNA sequence element required for efficient expression of a developmentally regulated and cAMP-inducible gene of Dictyostelium discoideum.
Pears CJ, Williams JG. Pears CJ, et al. EMBO J. 1987 Jan;6(1):195-200. doi: 10.1002/j.1460-2075.1987.tb04738.x. EMBO J. 1987. PMID: 16453747 Free PMC article. - Inducible gene expression of moricin, a unique antibacterial peptide from the silkworm (Bombyx mori).
Furukawa S, Tanaka H, Nakazawa H, Ishibashi J, Shono T, Yamakawa M. Furukawa S, et al. Biochem J. 1999 May 15;340 ( Pt 1)(Pt 1):265-71. Biochem J. 1999. PMID: 10229682 Free PMC article. - Antagonistic effects of signal transduction by intracellular and extracellular cAMP on gene regulation in Dictyostelium.
Endl I, Konzok A, Nellen W. Endl I, et al. Mol Biol Cell. 1996 Jan;7(1):17-24. doi: 10.1091/mbc.7.1.17. Mol Biol Cell. 1996. PMID: 8741836 Free PMC article.