Transit of epidermal growth factor through coated pits of the Golgi system - PubMed (original) (raw)
Transit of epidermal growth factor through coated pits of the Golgi system
M C Willingham et al. J Cell Biol. 1982 Jul.
Abstract
Using the direct conjugate of epidermal growth factor (EGF) and horseradish peroxidase, we have followed the entry of EGF into KB (human carcinoma) cells. EGF initially was found bound diffusely to the entire cell surface at 4 degrees C; on warming to 37 degrees C, EGF was found clustered in clathrin-coated pits on the plasma membrane in 1 min or less. Within 1-2 min at 37 degrees C, EGF began to accumulate in receptosomes within the cell and remained there for up to 10 min. At 10-13 min after warming to 37 degrees C, EGF was found in thin reticular membranous elements of the Golgi system, as well as concentrated in the clathrin-coated pits present on these membranes. By 15 min after warming, EGF began to be delivered to lysosomes located near the Golgi system. These findings suggest that clathrin-coated pits in the Golgi reticular system accumulate EGF before delivery to lysosomes.
Similar articles
- Down-regulation of the epidermal growth factor receptor in KB cells is due to receptor internalization and subsequent degradation in lysosomes.
Beguinot L, Lyall RM, Willingham MC, Pastan I. Beguinot L, et al. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2384-8. doi: 10.1073/pnas.81.8.2384. Proc Natl Acad Sci U S A. 1984. PMID: 6326124 Free PMC article. - Kinetics of transit of transferrin and epidermal growth factor through clathrin-coated membranes.
Hanover JA, Willingham MC, Pastan I. Hanover JA, et al. Cell. 1984 Dec;39(2 Pt 1):283-93. doi: 10.1016/0092-8674(84)90006-0. Cell. 1984. PMID: 6149810 - The morphologic pathway of binding and internalization of epidermal growth factor in cultured cells. Studies on A431, KB, and 3T3 cells, using multiple methods of labelling.
Willingham MC, Haigler HT, Fitzgerald DJ, Gallo MG, Rutherford AV, Pastan IH. Willingham MC, et al. Exp Cell Res. 1983 Jun;146(1):163-75. doi: 10.1016/0014-4827(83)90334-8. Exp Cell Res. 1983. PMID: 6190668 - Multiple pathways of exocytosis, endocytosis, and membrane recycling: validation of a Golgi route.
Farquhar MG. Farquhar MG. Fed Proc. 1983 May 15;42(8):2407-13. Fed Proc. 1983. PMID: 6404654 Review. - Endocytosis and membrane traffic in cultured cells.
Willingham MC, Pastan I. Willingham MC, et al. Recent Prog Horm Res. 1984;40:569-87. doi: 10.1016/b978-0-12-571140-1.50018-9. Recent Prog Horm Res. 1984. PMID: 6207568 Review. No abstract available.
Cited by
- Internalization and processing of transferrin and the transferrin receptor in human carcinoma A431 cells.
Hopkins CR, Trowbridge IS. Hopkins CR, et al. J Cell Biol. 1983 Aug;97(2):508-21. doi: 10.1083/jcb.97.2.508. J Cell Biol. 1983. PMID: 6309862 Free PMC article. - Down-regulation of the epidermal growth factor receptor in KB cells is due to receptor internalization and subsequent degradation in lysosomes.
Beguinot L, Lyall RM, Willingham MC, Pastan I. Beguinot L, et al. Proc Natl Acad Sci U S A. 1984 Apr;81(8):2384-8. doi: 10.1073/pnas.81.8.2384. Proc Natl Acad Sci U S A. 1984. PMID: 6326124 Free PMC article. - The lytic granules of natural killer cells are dual-function organelles combining secretory and pre-lysosomal compartments.
Burkhardt JK, Hester S, Lapham CK, Argon Y. Burkhardt JK, et al. J Cell Biol. 1990 Dec;111(6 Pt 1):2327-40. doi: 10.1083/jcb.111.6.2327. J Cell Biol. 1990. PMID: 2277062 Free PMC article. - Direct visualization of the phosphorylated epidermal growth factor receptor during its internalization in A-431 cells.
Carpentier JL, White MF, Orci L, Kahn RC. Carpentier JL, et al. J Cell Biol. 1987 Dec;105(6 Pt 1):2751-62. doi: 10.1083/jcb.105.6.2751. J Cell Biol. 1987. PMID: 2447100 Free PMC article. - Intracellular class II HLA antigens are accessible to transferrin-neuraminidase conjugates internalized by receptor-mediated endocytosis.
Cresswell P. Cresswell P. Proc Natl Acad Sci U S A. 1985 Dec;82(23):8188-92. doi: 10.1073/pnas.82.23.8188. Proc Natl Acad Sci U S A. 1985. PMID: 2999796 Free PMC article.
References
- Science. 1981 Oct 30;214(4520):504-9 - PubMed
- Proc Natl Acad Sci U S A. 1981 Nov;78(11):6967-71 - PubMed
- J Cell Biol. 1981 Dec;91(3 Pt 2):77s-103s - PubMed
- Cell. 1982 Apr;28(4):831-41 - PubMed
- Histochem J. 1977 Sep;9(5):525-51 - PubMed