Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells - PubMed (original) (raw)
Chromosomal mapping of the simian sarcoma virus onc gene analogue in human cells
D C Swan et al. Proc Natl Acad Sci U S A. 1982 Aug.
Abstract
The primate cell-derived transforming gene (v-sis) of simian sarcoma virus (SSV) is represented as a single copy marker within cellular DNAs of mammalian species including human. The human analogue of v-sis can be distinguished from its rodent counterparts by Southern blotting analysis of EcoRI-restricted DNAs. By testing for the presence of the human v-sis-related fragment, c-sis (human), in somatic cell hybrids possessing varying numbers of human chromosome, as well as in segregants of such hybrids, it was possible to assign c-sis to human chromosome 22.
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