The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector - PubMed (original) (raw)

The herpes simplex virus amplicon: a new eucaryotic defective-virus cloning-amplifying vector

R R Spaete et al. Cell. 1982 Aug.

Abstract

We have employed repeat units of herpes simplex virus (HSV) defective genomes to derive a cloning-amplifying vector (amplicon) that can replicate in eucaryotic cells in the presence of standard HSV helper virus. The design of the HSV amplicon system is based on the previous observation that cotransfection of cells with helper virus DNA and seed monomeric repeat units of HSV defective genomes results in the regeneration of concatemeric defective genomes composed of multiple reiterations of the seed repeats. Cotransfection of cells with helper virus DNA and chimeric repeat units containing bacterial plasmid pKC7 DNA resulted in the generation of defective genomes composed of reiterations of the seed HSV-pKC7 repeats. These chimeric defective genomes were packaged into virus particles and could be propagated in virus stocks, with the most enriched passages containing more than 90% chimeric defective genomes. Furthermore, monomeric chimeric repeat units could be transferred back and forth between bacteria and eucaryotic cells. A derivative vector constructed so as to contain several unique restriction enzyme sites could be potentially employed in the introduction of additional viral or eucaryotic DNA sequences into eucaryotic cells.

PubMed Disclaimer

Similar articles

• Herpes simplex virus amplicon: effect of size on replication of constructed defective genomes containing eucaryotic DNA sequences.
  Kwong AD, Frenkel N. Kwong AD, et al. J Virol. 1984 Sep;51(3):595-603. doi: 10.1128/JVI.51.3.595-603.1984. J Virol. 1984. PMID: 6088785 Free PMC article.
• Class I defective herpes simplex virus DNA as a molecular cloning vehicle in eucaryotic cells.
  Barnett JW, Eppstein DA, Chan HW. Barnett JW, et al. J Virol. 1983 Nov;48(2):384-95. doi: 10.1128/JVI.48.2.384-395.1983. J Virol. 1983. PMID: 6312096 Free PMC article.
• Analysis of two potential shuttle vectors containing herpes simplex virus defective DNA.
  Bear SE, Colberg-Poley AM, Court DL, Carter BJ, Enquist LW. Bear SE, et al. J Mol Appl Genet. 1984;2(5):471-84. J Mol Appl Genet. 1984. PMID: 6090565
• The history of the HSV amplicon: from naturally occurring defective genomes to engineered amplicon vectors.
  Frenkel N. Frenkel N. Curr Gene Ther. 2006 Jun;6(3):277-301. doi: 10.2174/156652306777591992. Curr Gene Ther. 2006. PMID: 16787181 Review.
• Minireview: the herpes simplex virus amplicon--a versatile defective virus vector.
  Frenkel N, Singer O, Kwong AD. Frenkel N, et al. Gene Ther. 1994;1 Suppl 1:S40-6. Gene Ther. 1994. PMID: 8542394 Review.

Cited by

• Advances in Alpha Herpes Viruses Vaccines for Human.
  Preda M, Manolescu LSC, Chivu RD. Preda M, et al. Vaccines (Basel). 2023 Jun 12;11(6):1094. doi: 10.3390/vaccines11061094. Vaccines (Basel). 2023. PMID: 37376483 Free PMC article. Review.
• An HSV-1-H129 amplicon tracer system for rapid and efficient monosynaptic anterograde neural circuit tracing.
  Xiong F, Yang H, Song YG, Qin HB, Zhang QY, Huang X, Jing W, Deng M, Liu Y, Liu Z, Shen Y, Han Y, Lu Y, Xu X, Holmes TC, Luo M, Zhao F, Luo MH, Zeng WB. Xiong F, et al. Nat Commun. 2022 Dec 10;13(1):7645. doi: 10.1038/s41467-022-35355-6. Nat Commun. 2022. PMID: 36496505 Free PMC article.
• Enhanced Production of Herpes Simplex Virus 1 Amplicon Vectors by Gene Modification and Optimization of Packaging Cell Growth Medium.
  Fernández-Frías I, Pérez-Luz S, Díaz-Nido J. Fernández-Frías I, et al. Mol Ther Methods Clin Dev. 2020 Mar 13;17:491-496. doi: 10.1016/j.omtm.2020.03.005. eCollection 2020 Jun 12. Mol Ther Methods Clin Dev. 2020. PMID: 32258212 Free PMC article.
• Toward Combined Cell and Gene Therapy for Genodermatoses.
  De Rosa L, Latella MC, Secone Seconetti A, Cattelani C, Bauer JW, Bondanza S, De Luca M. De Rosa L, et al. Cold Spring Harb Perspect Biol. 2020 May 1;12(5):a035667. doi: 10.1101/cshperspect.a035667. Cold Spring Harb Perspect Biol. 2020. PMID: 31653644 Free PMC article. Review.
• In vivo methods for acute modulation of gene expression in the central nervous system.
  Cwetsch AW, Pinto B, Savardi A, Cancedda L. Cwetsch AW, et al. Prog Neurobiol. 2018 Sep;168:69-85. doi: 10.1016/j.pneurobio.2018.04.008. Epub 2018 Apr 22. Prog Neurobiol. 2018. PMID: 29694844 Free PMC article. Review.

Publication types

MeSH terms

LinkOut - more resources

• Full Text Sources

  • Elsevier Science

• Other Literature Sources

  • The Lens - Patent Citations