Relationship between the amounts of EBV-DNA and EBNA per cell, clonability and tumorigenicity in two ebv-negative lymphoma lines and their EBV-converted sublines - PubMed (original) (raw)

Relationship between the amounts of EBV-DNA and EBNA per cell, clonability and tumorigenicity in two ebv-negative lymphoma lines and their EBV-converted sublines

I Ernberg et al. Int J Cancer. 1983.

Abstract

The effect of EBV-conversion of two EBV-negative lymphoma lines (Ramos and BJAB) on agarose clonability and tumorigenicity in nude mice was explored. The cloning frequency was increased in all 9 sublines investigated, between 1.1 and 4.9 times compared to the original "parental" lines. Tumorigenicity was increased in one out of 2 BJAB-derived, EBV-positive lines and in 3 of 6 Ramos-derived lines, while it was decreased in 3 others. A strong positive correlation between the number of genomes/cell and the amount of EBNA/cell was detected, and in 7 of 9 converted lines the cloning frequency also correlated to the number of EBV genomes. On the other hand, no relation was established between the number of EBV-genomes/cell, the amount of EBNA/cell and tumorigenicity.

PubMed Disclaimer

Similar articles

• Expression of the Epstein-Barr virus (EBV)-encoded membrane protein LMP1 impairs the in vitro growth, clonability and tumorigenicity of an EBV-negative Burkitt lymphoma line.
  Cuomo L, Ramquist T, Trivedi P, Wang F, Klein G, Masucci MG. Cuomo L, et al. Int J Cancer. 1992 Jul 30;51(6):949-55. doi: 10.1002/ijc.2910510619. Int J Cancer. 1992. PMID: 1322379
• Heterogeneity of Epstein-Barr virus derived from P3HR-1 cells.
  zur Hausen H, Fresen KO. zur Hausen H, et al. IARC Sci Publ (1971). 1978;(20):391-6. IARC Sci Publ (1971). 1978. PMID: 215522
• Production by EBV infection of an EBNA-positive subline from an EBNA-negative human lymphoma cell line without detectable EBV DNA.
  Clements GB, Klein G, Povey S. Clements GB, et al. Int J Cancer. 1975 Jul 15;16(1):125-33. doi: 10.1002/ijc.2910160114. Int J Cancer. 1975. PMID: 170210
• Transient induction of a nuclear antigen unrelated to Epstein-Barr nuclear antigen in cells of two human B-lymphoma lines converted by Epstein-Barr virus.
  Fresen KO, zur Hausen H. Fresen KO, et al. Proc Natl Acad Sci U S A. 1977 Jan;74(1):363-6. doi: 10.1073/pnas.74.1.363. Proc Natl Acad Sci U S A. 1977. PMID: 189313 Free PMC article.
• Segregation of the EBV-determined nuclear antigen (EBNA) in somatic cell hybrids derived from the fusion of a mouse fibroblast and a human Burkitt lymphoma line.
  Klein G, Wiener F, Zech L, zur Hausen H, Reedman B. Klein G, et al. Int J Cancer. 1974 Jul 15;14(1):54-64. doi: 10.1002/ijc.2910140108. Int J Cancer. 1974. PMID: 4376508 Review. No abstract available.

Cited by

• Overexpression of an activated REL mutant enhances the transformed state of the human B-lymphoma BJAB cell line and alters its gene expression profile.
  Chin M, Herscovitch M, Zhang N, Waxman DJ, Gilmore TD. Chin M, et al. Oncogene. 2009 May 21;28(20):2100-11. doi: 10.1038/onc.2009.74. Epub 2009 Apr 20. Oncogene. 2009. PMID: 19377508 Free PMC article.
• Epstein-Barr virus latent membrane protein (LMP1) and nuclear proteins 2 and 3C are effectors of phenotypic changes in B lymphocytes: EBNA-2 and LMP1 cooperatively induce CD23.
  Wang F, Gregory C, Sample C, Rowe M, Liebowitz D, Murray R, Rickinson A, Kieff E. Wang F, et al. J Virol. 1990 May;64(5):2309-18. doi: 10.1128/JVI.64.5.2309-2318.1990. J Virol. 1990. PMID: 2157887 Free PMC article.
• Epstein-Barr virus (EBV) recombinants: use of positive selection markers to rescue mutants in EBV-negative B-lymphoma cells.
  Wang F, Marchini A, Kieff E. Wang F, et al. J Virol. 1991 Apr;65(4):1701-9. doi: 10.1128/JVI.65.4.1701-1709.1991. J Virol. 1991. PMID: 1848303 Free PMC article.
• Induction of B cell responsiveness to growth factors by Epstein-Barr virus conversion: comparison of endogenous factors and interleukin-1.
  Blazar BA, Murphy AM. Blazar BA, et al. Clin Exp Immunol. 1990 Apr;80(1):62-8. doi: 10.1111/j.1365-2249.1990.tb06442.x. Clin Exp Immunol. 1990. PMID: 2157568 Free PMC article.

Publication types

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • Wiley