Ultrastructural immunocytochemical localization of the phosphomannosyl receptor in Chinese hamster ovary (CHO) cells - PubMed (original) (raw)
Ultrastructural immunocytochemical localization of the phosphomannosyl receptor in Chinese hamster ovary (CHO) cells
M C Willingham et al. J Histochem Cytochem. 1983 Jan.
Abstract
Using ultrastructural immunocytochemistry and antibodies directed against bovine liver phosphomannosyl (PM) receptor, we have localized the receptor in Chinese hamster ovary (CHO) cells. The majority of the receptor was found within the cell. Only a small fraction of the receptor was found on the surface and most of it was clustered in coated pits. Because these cells contain endogenous ligands for the receptor, it was not possible to determine if this clustered state was dependent on occupancy of the receptor. The bulk of the cell's receptor was found in the endoplasmic reticulum, nuclear envelope, and in the Golgi system. Most of the Golgi localization was associated with peripheral Golgi elements, suggesting a possible concentration of receptor in GERL. Very little receptor was found associated with mature lysosomes. PM receptor was also localized in structures that were identified as receptosomes by the presence of alpha 2-macroglobulin (alpha 2M)-gold, a ligand previously shown to enter CHO cells by the coated pit-receptosome pathway. This finding is consistent with the notion that during receptor-mediated endocytosis, receptors accompany ligand from the coated pit into the receptosome. The observation that the majority of the receptor was found in the endoplasmic reticulum and structures similar to GERL raises the possibility that the PM receptor plays an important role in compartmentalization of lysosomal enzymes in the GERL system.
Similar articles
- Morphologic study of the internalization of a lysosomal enzyme by the mannose 6-phosphate receptor in cultured Chinese hamster ovary cells.
Willingham MC, Pastan IH, Sahagian GG, Jourdian GW, Neufeld EF. Willingham MC, et al. Proc Natl Acad Sci U S A. 1981 Nov;78(11):6967-71. doi: 10.1073/pnas.78.11.6967. Proc Natl Acad Sci U S A. 1981. PMID: 6273898 Free PMC article. - Ultrastructural immunocytochemical localization of the transferrin receptor using a monoclonal antibody in human KB cells.
Willingham MC, Pastan I. Willingham MC, et al. J Histochem Cytochem. 1985 Jan;33(1):59-64. doi: 10.1177/33.1.2856926. J Histochem Cytochem. 1985. PMID: 2856926 - The receptosome: an intermediate organelle of receptor mediated endocytosis in cultured fibroblasts.
Willingham MC, Pastan I. Willingham MC, et al. Cell. 1980 Aug;21(1):67-77. doi: 10.1016/0092-8674(80)90115-4. Cell. 1980. PMID: 6157480 - Receptor-mediated endocytosis of alpha 2-macroglobulin in cultured fibroblasts.
Willingham MC, Maxfield FR, Pastan I. Willingham MC, et al. J Histochem Cytochem. 1980 Aug;28(8):818-23. doi: 10.1177/28.8.6160180. J Histochem Cytochem. 1980. PMID: 6160180 - The relationships between the Golgi apparatus, GERL, and lysosomes of fetal rat liver Kupffer cells examined by ultrastructural phosphatase cytochemistry.
Pino RM, Pino LC, Bankston PW. Pino RM, et al. J Histochem Cytochem. 1981 Sep;29(9):1061-70. doi: 10.1177/29.9.6116732. J Histochem Cytochem. 1981. PMID: 6116732
Cited by
- The insulin-like growth factor II/mannose-6-phosphate receptor : IGF-II/Man-6-P receptor.
Macdonald RG. Macdonald RG. Cytotechnology. 1989 Dec;2(4):287-305. doi: 10.1007/BF00364994. Cytotechnology. 1989. PMID: 22358870 - Endocytosed cation-independent mannose 6-phosphate receptor traffics via the endocytic recycling compartment en route to the trans-Golgi network and a subpopulation of late endosomes.
Lin SX, Mallet WG, Huang AY, Maxfield FR. Lin SX, et al. Mol Biol Cell. 2004 Feb;15(2):721-33. doi: 10.1091/mbc.e03-07-0497. Epub 2003 Oct 31. Mol Biol Cell. 2004. PMID: 14595110 Free PMC article. - Chimeric forms of furin and TGN38 are transported with the plasma membrane in the trans-Golgi network via distinct endosomal pathways.
Mallet WG, Maxfield FR. Mallet WG, et al. J Cell Biol. 1999 Jul 26;146(2):345-59. doi: 10.1083/jcb.146.2.345. J Cell Biol. 1999. PMID: 10465644 Free PMC article. - Transport from late endosomes to lysosomes, but not sorting of integral membrane proteins in endosomes, depends on the vacuolar proton pump.
van Weert AW, Dunn KW, Geuze HJ, Maxfield FR, Stoorvogel W. van Weert AW, et al. J Cell Biol. 1995 Aug;130(4):821-34. doi: 10.1083/jcb.130.4.821. J Cell Biol. 1995. PMID: 7642700 Free PMC article. - Ultrastructural localization of the mannose 6-phosphate receptor in rat liver.
Geuze HJ, Slot JW, Strous GJ, Hasilik A, Von Figura K. Geuze HJ, et al. J Cell Biol. 1984 Jun;98(6):2047-54. doi: 10.1083/jcb.98.6.2047. J Cell Biol. 1984. PMID: 6327724 Free PMC article.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources