Binding of epidermal growth factor in rat pancreatic acini - PubMed (original) (raw)
Binding of epidermal growth factor in rat pancreatic acini
M Korc et al. Biochem Biophys Res Commun. 1983.
Abstract
Specific, saturable EGF receptors were demonstrated in isolated rat pancreatic acini. Binding of EGF to these receptors was one-half maximal at 20 min and maximal at 120 min. Scatchard analyses revealed a single order of binding sites with a Kd of 4.90 nM. Following binding, EGF was rapidly internalized and converted to two acidic species. EGF did not alter either basal amylase release or the rate of [3H]phenylalanine incorporation into TCA-precipitable protein. The finding of high affinity EGF receptors in pancreatic acinar cells supports the hypothesis that EGF participates in the long-term regulation of pancreatic exocrine function.
Similar articles
- Epidermal growth factor binding is altered in pancreatic acini from diabetic rats.
Korc M, Matrisian LM, Nakamura R, Magun BE. Korc M, et al. Life Sci. 1984 Nov 12;35(20):2049-55. doi: 10.1016/0024-3205(84)90562-9. Life Sci. 1984. PMID: 6092811 - Epidermal growth factor receptor signaling in rat pancreatic acinar cells.
Stryjek-Kaminska D, Piiper A, Stein J, Caspary WF, Zeuzem S. Stryjek-Kaminska D, et al. Pancreas. 1995 Apr;10(3):274-80. doi: 10.1097/00006676-199504000-00009. Pancreas. 1995. PMID: 7542769 - Demonstration of epidermal growth factor binding sites in the adult rat pancreas by light microscopic autoradiography.
Chabot JG, Walker P, Pelletier G. Chabot JG, et al. Pancreas. 1987;2(6):653-7. doi: 10.1097/00006676-198711000-00005. Pancreas. 1987. PMID: 3501865 - Epidermal growth factor binding and biologic effects on mouse pancreatic acini.
Logsdon CD, Williams JA. Logsdon CD, et al. Gastroenterology. 1983 Aug;85(2):339-45. Gastroenterology. 1983. PMID: 6190700 - Receptors for cholecystokinin and insulin in isolated pancreatic acini: hormonal control of secretion and metabolism.
Williams JA, Sankaran H, Korc M, Goldfine ID. Williams JA, et al. Fed Proc. 1981 Aug;40(10):2497-502. Fed Proc. 1981. PMID: 6266879 Review.
Cited by
- Two subclasses of EGF receptors in the human pancreatic cancer cell lines CAPAN-1 and MIA PaCa-2.
Oustry P, Estival A, Pradayrol L, Vaysse N, Clémente F. Oustry P, et al. Int J Pancreatol. 1990 Mar;6(2):119-28. doi: 10.1007/BF02933046. Int J Pancreatol. 1990. PMID: 2230359 - Secretion of protein and epidermal growth factor (EGF) by transplanted human pancreas.
Konturek JW, Buesing M, Hopt UT, Stachura J, Becker HD, Konturek SJ. Konturek JW, et al. Int J Pancreatol. 1992 Aug;12(1):23-9. doi: 10.1007/BF02927067. Int J Pancreatol. 1992. PMID: 1527447 - Epidermal growth factor regulates adenylate cyclase activity via Gs and Gi1-2 proteins in pancreatic acinar membranes.
Stryjek-Kaminska D, Piiper A, Zeuzem S. Stryjek-Kaminska D, et al. Biochem J. 1996 May 15;316 ( Pt 1)(Pt 1):87-91. doi: 10.1042/bj3160087. Biochem J. 1996. PMID: 8645237 Free PMC article. - Characterization of epidermal growth factor receptors on plasma membranes isolated from rat gastric mucosa.
Hori R, Nomura H, Iwakawa S, Okumura K. Hori R, et al. Pharm Res. 1990 Jun;7(6):665-9. doi: 10.1023/a:1015838816061. Pharm Res. 1990. PMID: 2195496