Possible temperature-dependent blockage of synaptic vesicle recycling induced by a single gene mutation in Drosophila - PubMed (original) (raw)
Possible temperature-dependent blockage of synaptic vesicle recycling induced by a single gene mutation in Drosophila
T Kosaka et al. J Neurobiol. 1983 May.
Abstract
The temperature-sensitive Drosophila mutant, shibirets1 (shi), has been shown to exhibit a reversible block in synaptic transmission at 30 degrees C. Various synaptic terminals (neuromuscular, sensory, central) of this mutant were observed by electron microscopy. At 19 degrees C, all terminals of shi showed essentially the same structural features as those of wild-type (Oregon-R) flies, while at 30 degrees C (5 or 10 min of exposure) shi terminals exhibited various structural changes not seen in the wild type. The major structural change observed in all of the various types of terminals was the accumulation of many pitlike structures on the plasma membrane near presynaptic sites. These structures consisted of a spherical head portion, about 50-100 nm in diameter, and a cylindrical neck portion, about 20 nm long and 20-25 nm in diameter. The neck portion was surrounded by a kind of cytoplasmic dense material, about 10 nm thick, reminiscent of a "collar." Thus, these pits are referred to as "collared pits." Similar kinds of pits were observed, although very rarely, in wild-type flies at 19 and 30 degrees C and in shi flies at 19 degrees C. In addition, various degrees of vesicle depletion, and an increase in membranous structures (infoldings and cisternalike or tubulelike structures) often accompanied pit formation. The possibility that these pits are the result of a blocked step in the endocytotic process, which in turn causes vesicle depletion as exocytosis proceeds, is discussed.
Similar articles
- Drosophila synaptotagmin I null mutants show severe alterations in vesicle populations but calcium-binding motif mutants do not.
Loewen CA, Royer SM, Reist NE. Loewen CA, et al. J Comp Neurol. 2006 May 1;496(1):1-12. doi: 10.1002/cne.20868. J Comp Neurol. 2006. PMID: 16528727 - Evidence for a presynaptic blockage of transmission in a temperature-sensitive mutant of Drosophila.
Koenig JH, Ikeda K. Koenig JH, et al. J Neurobiol. 1983 Nov;14(6):411-9. doi: 10.1002/neu.480140602. J Neurobiol. 1983. PMID: 6139412 - Reversible control of synaptic transmission in a single gene mutant of Drosophila melanogaster.
Koenig JH, Saito K, Ikeda K. Koenig JH, et al. J Cell Biol. 1983 Jun;96(6):1517-22. doi: 10.1083/jcb.96.6.1517. J Cell Biol. 1983. PMID: 6304107 Free PMC article. - Synaptic vesicle pools and plasticity of synaptic transmission at the Drosophila synapse.
Kidokoro Y, Kuromi H, Delgado R, Maureira C, Oliva C, Labarca P. Kidokoro Y, et al. Brain Res Brain Res Rev. 2004 Dec;47(1-3):18-32. doi: 10.1016/j.brainresrev.2004.05.004. Brain Res Brain Res Rev. 2004. PMID: 15572160 Review. - Exocytosis and endocytosis of synaptic vesicles and functional roles of vesicle pools: lessons from the Drosophila neuromuscular junction.
Kuromi H, Kidokoro Y. Kuromi H, et al. Neuroscientist. 2005 Apr;11(2):138-47. doi: 10.1177/1073858404271679. Neuroscientist. 2005. PMID: 15746382 Review.
Cited by
- An approach for quantitative mapping of synaptic periactive zone architecture and organization.
Del Signore SJ, Mitzner MG, Silveira AM, Fai TG, Rodal AA. Del Signore SJ, et al. Mol Biol Cell. 2023 May 15;34(6):ar51. doi: 10.1091/mbc.E22-08-0372. Epub 2022 Dec 21. Mol Biol Cell. 2023. PMID: 36542486 Free PMC article. - Role of Clathrin and Dynamin in Clathrin Mediated Endocytosis/Synaptic Vesicle Recycling and Implications in Neurological Diseases.
Prichard KL, O'Brien NS, Murcia SR, Baker JR, McCluskey A. Prichard KL, et al. Front Cell Neurosci. 2022 Jan 18;15:754110. doi: 10.3389/fncel.2021.754110. eCollection 2021. Front Cell Neurosci. 2022. PMID: 35115907 Free PMC article. Review. - Optogenetics in Drosophila.
Kohsaka H, Nose A. Kohsaka H, et al. Adv Exp Med Biol. 2021;1293:309-320. doi: 10.1007/978-981-15-8763-4_19. Adv Exp Med Biol. 2021. PMID: 33398822 Review. - Phagocytic glia are obligatory intermediates in transmission of mutant huntingtin aggregates across neuronal synapses.
Donnelly KM, DeLorenzo OR, Zaya AD, Pisano GE, Thu WM, Luo L, Kopito RR, Panning Pearce MM. Donnelly KM, et al. Elife. 2020 May 28;9:e58499. doi: 10.7554/eLife.58499. Elife. 2020. PMID: 32463364 Free PMC article. - A Novel Neuroprotective Role of Phosphatase of Regenerating Liver-1 against CO2 Stimulation in Drosophila.
Guo P, Xu X, Wang F, Yuan X, Tu Y, Zhang B, Zheng H, Yu D, Ge W, Gong Z, Yang X, Xi Y. Guo P, et al. iScience. 2019 Sep 27;19:291-302. doi: 10.1016/j.isci.2019.07.026. Epub 2019 Jul 22. iScience. 2019. PMID: 31404830 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases